| 货号 | AF3004-SP |
| 别名 | LCA-homolog; protein tyrosine phosphatase, receptor type, F; PTPRF; receptor type, F polypeptide; receptor-linked protein-tyrosine phosphatase LAR; receptor-type tyrosine-protein phosphatase F | 全称 | Leukocyte Antigen-Related Tyrosine Phosphatase |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human, mouse, and rat LAR in Western blots. In Western blots, this antibody detects both full-length protein and the cleaved LAR extracellular (E) domain. The antibody does not cross react with PTPR sigma. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5792 (Human); 104121 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human LAR Ala27-Glu1251 Accession # NP_569707 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Leucocyte Antigen-Related (LAR) tyrosine phosphatase, also known as Protein Tyrosine Phoshatase, Receptor-type F (PTPRF), is an integral membrane protein with anon-glycosylated molecular weight of 207 kDa. The extracellular domain is cleaved near the cell membrane by a subtilisin-like endoprotease to a molecular weight of 139 kDa. Depending on cellular conditions, the extracellular domain may remain associated with the rest of the molecule or can be shed into the extracellular medium. This antibody was made against the extracellular domain of LAR and detects both full-length and cleaved LAR. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse/Rat LAR by Western Blot. Western blot shows lysates of Raji human Burkitts lymphoma cell line, Neuro‑2A mouse neuroblastoma cell line, and Y3‑Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat LAR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3004) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Bands were detected for LAR at approximately 207 kDa for full-length LAR and 139 kDa for cleaved LAR (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
