Human/Mouse/Rat HIF-2 alpha/EPAS1 Affinity Purified PAb (25 UG)【科瑞奥博】

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Human/Mouse/Rat HIF-2 alpha/EPAS1 Affinity Purified PAb (25 UG)

货号： AF2997-SP 基本售价： 1407.8 元规格： -

产品信息

概述
货号	AF2997-SP
别名	Basic-helix-loop-helix-PAS protein MOP2; BHLHE73; bHLHe73ECYT4; Class E basic helix-loop-helix protein 73; endothelial PAS domain protein 1; endothelial PAS domain-containing protein 1; EPAS1; EPAS-1; HIF-1alpha-like factor; HIF-2 alpha; hif2a angiogenesis; HIF2A; HIF2AHIF-2-alpha; HLF; HLFHIF-1-alpha-like factor; hypoxia-inducible factor 2 alpha; Hypoxia-inducible factor 2-alpha; Member of PAS protein 2; MOP2; MOP2HIF2-alpha; PASD2PAS domain-containing protein 2
全称	Hypoxia-inducible Transcription Factor 2 alpha
反应种属	Human/Mouse/Rat
应用	Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human, mouse, and rat HIF-2 alpha /EPAS1 in Western blots.
使用方法	Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2034 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Distinct subpopulations of FOXD1 stroma-derived cells regulate renal erythropoietin Authors: H Kobayashi, Q Liu, TC Binns, AA Urrutia, O Davidoff, PP Kapitsinou, AS Pfaff, H Olauson, A Wernerson, AB Fogo, GH Fong, KW Gross, VH Haase J Clin Invest, 2016;0(0):. Species: Mouse Sample Type: Tissue Homogenates Application: WB Unprocessed Interleukin-36alpha Regulates Psoriasis-Like Skin Inflammation in Cooperation With Interleukin-1. Authors: Milora K, Fu H, Dubaz O, Jensen L J Invest Dermatol, 2015;135(12):2992-3000. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development Prolyl-4-hydroxylase domain 3 (PHD3) is a critical terminator for cell survival of macrophages under stress conditions. Authors: Swain L, Wottawa M, Hillemann A, Beneke A, Odagiri H, Terada K, Endo M, Oike Y, Farhat K, Katschinski D J Leukoc Biol, 2014;96(3):365-75. Species: Mouse Sample Type: Cell Lysates Application: WB IL-4 reduces the proangiogenic capacity of macrophages by down-regulating HIF-1alpha translation. Authors: Dehne N, Tausendschon M, Essler S, Geis T, Schmid T, Brune B J Leukoc Biol, 2014;95(1):129-37. Species: Human Sample Type: Cell Lysate Application: WB HIF-1alpha activation results in actin cytoskeleton reorganization and modulation of Rac-1 signaling in endothelial cells. Authors: Weidemann A, Breyer J, Rehm M, Eckardt K, Daniel C, Cicha I, Giehl K, Goppelt-Struebe M Cell Commun Signal, 2013;11(0):80. Species: Mouse Sample Type: Cell Lysates Application: WB Hypoxia-inducible Factor-1 (HIF-1) but Not HIF-2 Is Essential for Hypoxic Induction of Collagen Prolyl 4-Hydroxylases in Primary Newborn Mouse Epiphyseal Growth Plate Chondrocytes. Authors: Aro E, Khatri R J. Biol. Chem., 2012;287(44):37134-44. Species: Mouse Sample Type: Cell Lysates Application: WB The hypoxic microenvironment upgrades stem-like properties of ovarian cancer cells. Authors: Liang D, Ma Y, Liu J, Trope CG, Holm R, Nesland JM, Suo Z BMC Cancer, 2012;12(0):201. Species: Human Sample Type: Cell Lysates Application: WB Loss of myeloid cell-derived vascular endothelial growth factor accelerates fibrosis. Authors: Stockmann C, Kerdiles Y, Nomaksteinsky M, Weidemann A, Takeda N, Doedens A, Torres-Collado AX, Iruela-Arispe L, Nizet V, Johnson RS Proc. Natl. Acad. Sci. U.S.A., 2010;107(9):4329-34. Species: Mouse Sample Type: Tissue Homogenates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse HIF-2 alpha /EPAS1 Ser542-Thr874 Accession # AAH57870
生物活性	Human, Mouse, Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Thehypoxia-inducible transcription factor2 alpha (HIF-2 alpha ) is stabilized in hypoxic tissue and, similarly to HIF-1 alpha, complexes with Aryl hydrocarbon receptor nuclear translocator (ARNT). Both the HIF-1 and HIF-2 complexes bind hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. HIF-1 and HIF-2 do not appear completely redundant, although specific functions are only beginning to be elucidated. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the potentially broad impact of gene regulation by both HIF-1 alpha and HIF-2 alpha.
运输条件	Blue Ice
存放说明	4℃
参考文献	Basic Helix-Loop-Helix (bHLH) Transcription Factors Cancer Biomarkers Cancer Stem Cell Markers Cancer Stem Cell Transcription Factors HIF Transcription Factors Intracellular Signaling Molecules in Angiogenesis Transcription Factors in the Akt Pathway Transcriptional Regulators in Allergy and Asthma

参考图片

HIF‑2 alpha /EPAS1 in Human Prostate Cancer Tissue. HIF‑2 alpha /EPAS1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human/Mouse/Rat HIF‑2 alpha /EPAS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2997) at 0.3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

HIF‑2 alpha /EPAS1 in Mouse Testis. HIF‑2 alpha /EPAS1 was detected in perfusion fixed frozen sections of mouse testis using Goat Anti-Human/Mouse/Rat HIF‑2 alpha /EPAS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2997) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei in spermatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Human, Mouse, and Rat
HIF‑2 alpha /EPAS1 by Western Blot. Western blot shows lysates of
MCF‑7 human breast cancer cell line, bEnd.3 mouse endothelioma cell line, and PC‑12 rat adrenal pheochromocytoma cell line untreated (-) or treated (+) with 150 µM CoCl₂ for 16 hours. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse HIF‑2 alpha /EPAS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2997) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HIF‑2 alpha /EPAS1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.