Human Carbonic Anhydrase XII Affinity Purified PAb (25 UG)【科瑞奥博】

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Human Carbonic Anhydrase XII Affinity Purified PAb (25 UG)

货号： AF2190-SP 基本售价： 1407.8 元规格： -

产品信息

概述
货号	AF2190-SP
别名	CA12; Carbonate dehydratase XII; carbonic anhydrase 12; carbonic anhydrase XIICAXII; carbonic dehydratase; CA-XII; EC 4.2.1.1; FLJ20151; HsT18816; Tumor antigen HOM-RCC-3.1.3
反应种属	Human
应用	Western Blot(1 µg/mL)
目标/特异性	Detects human Carbonic Anhydrase XII/CA12 in direct ELISAs and Western blots. In Western blots, approximately 40% cross-reactivity with recombinant mouse Carbonic Anhydrase XII/CA12 is observed and less than 5% cross-reactivity with recombinant human CA1, 3, 5A, 5B, 6, 7, 8, and 10 is observed.
使用方法	Western Blot: 1 µg/mL Simple Western: 10 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	771 (Human); 76459 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Carbonic anhydrase IX promotes tumor growth and necrosis in vivo and inhibition enhances anti-VEGF therapy. Authors: McIntyre A, Patiar S, Wigfield S Clin. Cancer Res., 2012;18(11):3100-11. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase XII/CA12 Ala25-Gln291 Accession # O43570
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6^th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO₂ removal and mosquito control in industry. CA12 is a type I membrane enzyme highly expressed in colon, kidney, prostate, intestine and activated lymphocytes and moderately expressed in pancreas, ovary, and testis (2, 3). It is over-expressed in some renal cell cancers and in glaucoma (2, 4). Two alternatively spliced forms exist, which either contains or lacks a 11 amino acid segment just before the transmembrane domain (5). The secreted, purified recombinant human CA12 contains the residues that are common for both forms.
运输条件	Blue Ice
存放说明	4℃
参考文献	Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50. Tuereci, O. et al. (1998) Proc. Natl. Acad. Sci. USA 95:7608. Ivanov, S.V. et al. (1998) Proc. Natl. Acad. Sci. USA 95:12596. Liao, S.-Y. et al. (2003) J. Med. Genet. 40:257. Strausberg, R.L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:16899.

参考图片

Detection of Human Carbonic Anhydrase XII/CA12 by Western Blot. Western blot shows lysates of human kidney tissue and A549 human lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Carbonic Anhydrase XII/CA12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2190) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Carbonic Anhydrase XII/CA12 at approximately
45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.