Human Carbonic Anhydrase VIII Affinity Purified PAb (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human Carbonic Anhydrase VIII Affinity Purified PAb (25 UG)

货号： AF2187-SP 基本售价： 1407.8 元规格： -

产品信息

概述
货号	AF2187-SP
别名	CA8; CALS; CALScarbonate dehydratase; CAMRQ3; carbonic anhydrase VIIIMGC99509; carbonic anhydrase-like sequence; carbonic anhydrase-related protein; CARP; CARPCA-related protein; CA-VIII; MGC120502
反应种属	Human
应用	Western Blot(0.5 µg/mL) Simple Western(5 µg/mL) Immunoprecipitation(25 µg/mL)
目标/特异性	Detects human Carbonic Anhydrase VIII/CA8 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) CA2 and rhCA3 is observed and less than 1% cross-reactivity with rhCA1, rhCA4, and rhCA9 is observed.
使用方法	Western Blot: 0.5 µg/mL Simple Western: 5 µg/mL Immunoprecipitation: 25 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	767 (Human)
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human Carbonic Anhydrase VIII/CA8 Ala2-Gln290 Accession # P35219
生物活性	Human, Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6^th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO₂ removal and mosquito control in industry. CA8, also called CA‑related protein (CARP), is a cytosolic protein without CA activity (i.e., the reversible hydration of CO₂) due to point mutations in the zinc-binding site (2). Nevertheless, it has esterase activity described in the Activity Assay Protocol. CA8 is expressed exclusively in Purkinje cells of the cerebellum, where it binds inositol 1,4,5‑triphosphate receptor type 1 (3). CA8 overexpression in human colorectal cancer and non-small cell lung cancer indicates that it plays a role in the process of invasion in these types of malignancy (4, 5).
运输条件	Blue Ice
存放说明	4℃
参考文献	Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50. Sjoblom, J. et al. (1996) FEBS Lett. 398: 322. Hirota, J. et al. (2003) Biochem. J. 372: 435. Miyaji, E. et al. (2003) J. Pathol. 201: 37. Lu, S.H. et al. (2004) Lung Cancer 44: 273.

参考图片

Detection of Human and Mouse Carbonic Anhydrase VIII/CA8 by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue and mouse brain (cerebellum) tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Carbonic Anhydrase VIII/CA8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2187) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Carbonic Anhydrase VIII/CA8 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human and Mouse Carbonic Anhydrase VIII/CA8 by Simple Western^TM. Simple Western lane view shows lysates of human brain (cerebellum) tissue and mouse brain (cerebellum) tissue, loaded at 0.2 mg/mL. Specific bands were detected for Carbonic Anhydrase VIII/CA8 at approximately 41 and 50 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse Carbonic Anhydrase VIII/CA8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2187) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.