Human Carbonic Anhydrase III Affinity Purified PAb (25 UG)【科瑞奥博】

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Human Carbonic Anhydrase III Affinity Purified PAb (25 UG)

货号： AF2185-SP 基本售价： 1407.8 元规格： -

产品信息

概述
货号	AF2185-SP
别名	CA3; CAIII; CA-III; Car3; Carbonate dehydratase III; carbonic anhydrase 3; Carbonic anhydrase III; carbonic anhydrase III, muscle specific; EC 4.2.1.1
反应种属	Human
应用	Western Blot(0.1 µg/mL) Immunoprecipitation(25 µg/mL)
目标/特异性	Detects human Carbonic Anhydrase III/CA3 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) CA1, rhCA2, rhCA4, rhCA9, rhCA10, rhCA13 and rhCA14 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunoprecipitation: 25 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	761 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Comprehensive proteomics analysis of autophagy-deficient mouse liver. Authors: Matsumoto N, Ezaki J, Komatsu M, Takahashi K, Mineki R, Taka H, Kikkawa M, Fujimura T, Takeda-Ezaki M, Ueno T, Tanaka K, Kominami E Biochem. Biophys. Res. Commun., 2008;368(3):643-9. Species: Mouse Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human Carbonic Anhydrase III/CA3 Ala2-Lys260 Accession # P07451
生物活性	Human, Mouse, Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6^th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO₂ removal and mosquito control in industry. CA3 is a cytosolic enzyme with a very low CA activity. It is expressed at low levels in human muscle during early development but increases rapidly during the last trimester to reach 50‑60% of adult levels at birth (2).
运输条件	Blue Ice
存放说明	4℃
参考文献	Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50. Sly, W.S. and P.Y. Hu (1995) Annu. Rev. Biochem. 64:375.

参考图片

Detection of Human, Mouse, and Rat Carbonic Anhydrase III/CA3 by Western Blot. Western blot shows lysates of human, mouse, and rat skeletal muscle tissue and heart tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse/
Rat Carbonic Anhydrase III/CA3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2185) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Carbonic Anhydrase III/CA3 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.