| 货号 | AF1785-SP |
| 别名 | chromosome 8 open reading frame 57; DKFZp761D112; EC 3.4.24; EC 3.4.24.-; EC 3.4.24.80; matrix metallopeptidase 16 (membrane-inserted); matrix metalloproteinase 16 (membrane-inserted); matrix metalloproteinase-16; Membrane-type matrix metalloproteinase 3; Membrane-type-3 matrix metalloproteinase; MMP-16; MMPX2; MMP-X2; MT3MMP; MT3-MMP; MT3-MMPC8orf57; MT-MMP 3; MT-MMP2; MTMMP3; MT-MMP3; Putative transmembrane protein C8orf57 | 全称 | Matrix Metalloproteinase 16/Membrane Type 3 MMP |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects human MMP‑16/MT3‑MMP in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunoprecipitation: 25 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4325 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human MMP‑16/MT3‑MMP Ala32-Gly291 (Ile152Asn) Accession # P51512 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix (ECM). MMP-16 (MT3-MMP) is found in brain, lung, placenta, smooth muscle cells, and malignant tumor tissues including oral melanoma and renal carcinoma (1). MMP-16 has been shown to activate proMMP-2 and degrade various ECM components including native collagens (2, 3). MMP-16 has been proposed to possess the potential to directly enhance the growth and invasiveness of cells in vivo, two critical processes for development and carcinogenesis (4). Structurally, MMP-16 consists of the following domains: a pro domain containing the furin cleavage site, a catalytic domain containing the zinc-binding site, a hinge region, a hemopexin-like domain, a transmembrane domain, and a cytoplamasic tail (1). The structure of the catalytic domain in complex with a hydroxamate inhibitor has been solved (5). The rhMMP-16PC consists of the pro and catalytic domains, which can be activated by treatment with furin. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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MMP‑16/MT3‑MMP in Human Breast. MMP‑16/MT3‑MMP was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human MMP‑16/MT3‑MMP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1785) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
