Human Trappin-2/Elafin Affinity Purified PAb (25 UG)【科瑞奥博】

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Human Trappin-2/Elafin Affinity Purified PAb (25 UG)

货号： AF1747-SP 基本售价： 1407.8 元规格： -

产品信息

概述
货号	AF1747-SP
别名	cementoin; Elafin; Elastase-specific inhibitor; ESI; ESIWAP four-disulfide core domain protein 14; Peptidase inhibitor 3; peptidase inhibitor 3, skin-derived; PI3; PI-3; pre-elafin; protease inhibitor 3, skin-derived (SKALP); Protease inhibitor WAP3; SKALP; SKALPELAFIN; Skin-derived antileukoproteinase; trappin-2; WAP four-disulfide core domain 14; WAP3MGC13613; WFDC14elafin
全称	Elastase Inhibitor, Transglutaminase Substrate, Serine Protease
反应种属	Human
应用	Western Blot,Immunoprecipitation,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects human Trappin‑2/Elafin in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) SLPI, rhTGM-2, and human Neutrophil Elastase is observed.
使用方法	Western Blot: 0.1 µg/mL Immunoprecipitation: 25 µg/mL ELISA Capture (Matched Antibody Pair): 0.2-0.8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize Recombinant Human Trappin-2/ Elafin (1 µg/mL, Catalog # 1747-PI) inhibition of Recombinant Mouse Neutrophil Elastase/ELA2 (1 µg/mL, Catalog # 4517-SE) cleavage of the fluorogenic peptide substrate MeOSuc-AAPV-Amc (100 µM). The Neutralization Dose (ND₅₀) is typically 15.6 µg/mL.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5266 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. A systems biology examination of the human female genital tract shows compartmentalization of immune factor expression. Authors: Burgener, Adam, Tjernlund, Annelie, Kaldensjo, Tove, Abou, Max, McCorrister, Stuart, Westmacott, Garrett, Mogk, Kenzie, Ambrose, Emma, Broliden, Kristina, Ball, Blake J Virol, 2013;87(9):5141-50. Species: Human Sample Type: Whole Tissue Application: IHC Frozen Anti-HIV-1 activity of elafin is more potent than its precursor's, trappin-2, in genital epithelial cells. Authors: Drannik AG, Nag K, Yao XD, Henrick BM, Jain S, Ball TB, Plummer FA, Wachihi C, Kimani J, Rosenthal KL J. Virol., 2012;86(8):4599-610. Species: Human Sample Type: cervicovaginal lavage Application: Immunodepletion Elafin, an Elastase-specific Inhibitor, Is Cleaved by Its Cognate Enzyme Neutrophil Elastase in Sputum from Individuals with Cystic Fibrosis. Authors: Guyot N, Butler MW, McNally P, Weldon S, Greene CM, Levine RL, ONeill SJ, Taggart CC, McElvaney NG J. Biol. Chem., 2008;283(47):32377-85. Species: Human Sample Type: Sputum Application: ELISA Development
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human Trappin‑2/Elafin Ala23-Gln117 Accession # P19957
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Trappin-2 is the human member of the trappin gene family that contains SLPI (1). Trappin-2 consists of an N-terminal transglutaminase substrate domain (residues 23‑60) and a C-terminal four-disulfide core or whey acidic protein (WAP) domain (residues 72‑117). Elafin or ESI (elastase-specific inhibitor) and SKALP (skin-derived anti-leucoproteinase) are alternative names for Trappin-2 and reflect its protease targets. However, elafin and SKALP sometimes correspond only to the processed form that contains the C-terminal WAP domain of the molecule, which can be isolated naturally. The recombinant human Trappin-2 corresponds to the full‑length form (residues 23‑117), which migrates as two protein bands under SDS-PAGE due to an unidentified mechanism. In addition to its ability to inhibit human neutrophil elastase, it can also be used as a substrate for transglutaminases.
运输条件	Blue Ice
存放说明	4℃
参考文献	Schalkwijk, J. et al. (1999) Biochem. J. 340:569.

参考图片

Neutralization of Trappin‑2/
Elafin Activity by Human Trappin‑2/Elafin Antibody. Recombinant Mouse Neutrophil Elastase/ELA2 (1 µg/mL, Catalog # 4517-SE) activity is measured in the presence of Recombinant Human Trappin-2/ Elafin (1 µg/mL, Catalog # 1747‑PI) that has been preincubated with increasing concentrations of Goat Anti-Human Trappin‑2/Elafin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1747). The ND₅₀ is typically 15.6 µg/mL.