货号 | AF1644-SP |
别名 | EC 2.7.11; ERK3; ERK-6; ERK6MAPK 12; Extracellular signal-regulated kinase 6; MAP kinase 12; MAP kinase p38 gamma; MAPK12; mitogen-activated protein kinase 12; mitogen-activated protein kinase 3; Mitogen-activated protein kinase p38 gamma; p38gamma; PRKM12; SAPK-3; SAPK3EC 2.7.11.24; Stress-activated protein kinase 3 |
反应种属 | Human/Mouse/Rat |
应用 | Western Blot(0.2 µg/mL) |
目标/特异性 | Detects human, mouse, and rat p38 gamma in Western blots. The antibody is highly specific for p38 gamma and in Western blots, shows extremely weak cross-reactivity with recombinant p38 alpha, p38 beta, or p38δ after prolonged exposure to film. |
使用方法 | Western Blot: 0.2 µg/mL |
来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 6300 (Human) |
纯化方式 | Antigen Affinity-purified |
免疫原 | E. coli-derived recombinant human p38 gamma Met1-Leu367 Accession # P53778 |
生物活性 | Human, Mouse, Rat |
标记 | Unconjugated |
溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
背景 | p38 mitogen-activated protein kinase (MAPK)gamma, also known as p38 gamma, MAPK12, SAPK3 and ERK6 is a serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays a role in myoblast differentiation and also in the down-regulation of cyclin D1 in response to hypoxia in adrenal cells. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of Human, Mouse, and Rat p38 gamma by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line myoblast cell line and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.2 µg/mL Goat Anti-Human/Mouse/Rat p38 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1644) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human p38 beta, p38 gamma, p38δ and p38 alpha (5 ng/lane) were included. A specific band for p38 gamma was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |