Human CD163 Affinity Purified PAb (25 UG)【科瑞奥博】

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Human CD163 Affinity Purified PAb (25 UG)

货号： AF1607-SP 基本售价： 1407.8 元规格： -

产品信息

概述
货号	AF1607-SP
别名	CD163 antigenscavenger receptor cysteine-rich type 1 protein M130; CD163 molecule; CD163; GHI/61; HbSR; Hemoglobin scavenger receptor; M130; M130macrophage-associated antigen; MM130; RM3/1
反应种属	Human
应用	Western Blot(1 µg/mL) Simple Western(10 µg/mL)
目标/特异性	Detects human CD163 in direct ELISAs and Western blots.
使用方法	Western Blot: 1 µg/mL Simple Western: 10 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	9332 (Human); 93671 (Mouse); 312701 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Simian hemorrhagic fever virus cell entry is dependent on CD163 and uses a clathrin-mediated endocytosis-like pathway. Authors: Cai Y, Postnikova E, Bernbaum J, Yu S, Mazur S, Deiuliis N, Radoshitzky S, Lackemeyer M, McCluskey A, Robinson P, Haucke V, Wahl-Jensen V, Bailey A, Lauck M, Friedrich T, OConnor D, Goldberg T, Jahrling P, Kuhn J J Virol, 2015;89(1):844-56. Species: primate - grivet monkey Sample Type: Whole Cells Application: Neut Interaction of the European genotype porcine reproductive and respiratory syndrome virus (PRRSV) with sialoadhesin (CD169/Siglec-1) inhibits alveolar macrophage phagocytosis. Authors: De Baere MI, Van Gorp H, Delputte PL Vet. Res., 2012;43(1):47. Species: Porcine Sample Type: Whole Cells Application: Neut Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction. Authors: Zizzo G, Hilliard B, Monestier M, Cohen P J Immunol, 2012;189(7):3508-20. Species: Human Sample Type: Whole Cells Application: ICC Identification of the CD163 protein domains involved in infection of the porcine reproductive and respiratory syndrome virus. Authors: Van Gorp H, Van Breedam W, Van Doorsselaere J, Delputte PL, Nauwynck HJ J. Virol., 2010;84(6):3101-5. Species: Porcine Sample Type: Whole Cells Application: WB Availability of activated CD4+ T cells dictates the level of viremia in naturally SIV-infected sooty mangabeys. Authors: Klatt NR, Villinger F, Bostik P, Gordon SN, Pereira L, Engram JC, Mayne A, Dunham RM, Lawson B, Ratcliffe SJ, Sodora DL, Else J, Reimann K, Staprans SI, Haase AT, Estes JD, Silvestri G, Ansari AA J. Clin. Invest., 2008;118(6):2039-49. Species: Primate â€“ Cercocebus torquatus (Sooty Mangabey) Sample Type: Whole Tissue Application: IHC Paraffin-embedded Sialoadhesin and CD163 join forces during entry of the porcine reproductive and respiratory syndrome virus. Authors: Van Gorp H, Van Breedam W, Delputte PL, Nauwynck HJ J. Gen. Virol., 2008;89(0):2943-53. Species: Porcine Sample Type: Whole Cells Application: ICC CD163 expression confers susceptibility to porcine reproductive and respiratory syndrome viruses. Authors: Calvert JG, Slade DE, Shields SL, Jolie R, Mannan RM, Ankenbauer RG, Welch SK J. Virol., 2007;81(14):7371-9. Species: Primate - Macaca mulatta (Rhesus monkey) Sample Type: Whole Cells Application: Neut
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human CD163 (R&D Systems, Catalog # 1607-CD) Gly41-Ser1045 Accession # CAA80543
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	CD163, previously called M130 or p155, is a 130-160 kDa type I transmembrane glycoprotein that belongs to group B of the cysteine-rich scavenger receptor family (1-3). It is essential for clearance of hemoglobin-haptoglobin (Hb-Hp) complexes in the liver, spleen and circulation (4). The human CD163 contains a 41 amino acid (aa) signal sequence, a 1009 aa extracellular domain (ECD) with 9 scavenger receptor cysteine-rich (SRCR) domains, a 22 aa transmembrane segment, and a 39‑84 aa cytoplasmic region (1). The third SRCR domain is crucial for calcium-dependent binding of hemoglobin/haptoglobin complexes (3). Three splice forms (isoforms 2, 3 and 4) vary within their intracellular regions (1, 5), while one isoform (# 4) also has a 34 aa insert between SRCR domains 5 and 6 within the ECD. While all are expressed, isoform 3 is the most abundant, being generally expressed on the cell surface and most active in endocytosis (5). An approximately 130 kDa soluble form of human CD163 (sCD163) is assumed to contain virtually all of the ECD, which shares 74%, 75%, 84%, 86%, 86%, and 87% aa identity with mouse, rat, bovine, equine, porcine and canine CD163 ECD, respectively (6, 7). It is released from the cell surface by proteolysis after oxidative stress or inflammatory stimuli, including bacterial endotoxins and activation of the Toll-like receptors TLR2 or TLR5 (7 - 10). Expression of CD163 is constitutive, and induced by glucocorticoids, IL-10, IL-6 or endotoxin on circulating monocytes, tissue macrophages, and at low levels on monocyte-derived dendritic cells (1, 2, 11, 12). In addition to clearing Hb-Hp complexes, CD163 is also a scavenger receptor for free Hb (if Hp is depleted) and TWEAK (TNF-like weak inducer of apoptosis), and can function as an erythroblast adhesion receptor (4, 13-15).
运输条件	Blue Ice
存放说明	4℃
参考文献	Law, S.K.A. et al. (1993) Eur. J. Immunol. 23:2320. Sulahian, T.H. et al. (2000) Cytokine 12:1312. Madsen, M. et al. (2004) J. Biol. Chem. 279:51561. Kristiansen, M. et al. (2001) Nature 409:198. Nielsen, M.J. et al. (2006) J. Leukoc. Biol. 79:837. Moller, H.J. et al. (2002) Blood 99:378. Droste, A. et al. (1999) Biochem. Biophys. Res. Commun. 256:110. Hintz, K. A. et al. (2002) J. Leukoc. Biol. 72:711. Weaver, L.K. et al. (2006) J. Leukoc. Biol. 80:26. Timmerman, M. and P. Hogger (2005) Free Radic. Biol. Med. 39:98. Buechler, C. et al. (2000) J. Leukoc. Biol. 67:97. Pulford, K.A. et al. (1989) J. Clin. Pathol. 42:414. Schaer, D.J. et al. (2006) Blood 107:373. Bover, L.C. et al. (2007) J. Immunol. 178:8183. Fabriek, B.O. et al. (2007) Blood 109:5223.

参考图片

Detection of Human CD163 by Western Blot. Western blot shows lysates of human thymus tissue, human liver tissue, and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD163 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1607) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CD163 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human CD163 by Simple Western^TM. Simple Western lane view shows lysates of human placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for CD163 at approximately 179 kDa (as indicated) using 10 µg/mL of Goat Anti-Human CD163 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1607) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.