Human/Mouse/Rat JNK Pan Specific Affinity Purified PAb (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human/Mouse/Rat JNK Pan Specific Affinity Purified PAb (25 UG)

货号： AF1387-SP 基本售价： 1407.8 元规格： -

产品信息

概述
货号	AF1387-SP
别名	C-jun-amino-terminal kinase interacting protein 3; C-Jun-amino-terminal kinase-interacting protein 3; DKFZp762N1113; homolog of Drosophila Sunday driver 2; JIP-3; JIP3FLJ00027; JNK MAP kinase scaffold protein 3; JNK/SAPK-associated protein-1; JNK/stress-activated protein kinase-associated protein 1; JNK-interacting protein 3; JSAP1; KIAA1066SYD2; mitogen-activated protein kinase 8 interacting protein 3; Mitogen-activated protein kinase 8-interacting protein 3; syd
全称	C-Jun N-terminal Kinase
反应种属	Human/Mouse/Rat
应用	Western Blot(0.2 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human, mouse, and rat p46 and p54 JNK in Western blots. Detects recombinant human (rh) JNK1, rhJNK2, and rhJNK3.
使用方法	Western Blot: 0.2 µg/mL Immunohistochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. White-to-brown metabolic conversion of human adipocytes by JAK inhibition. Authors: Moisan A, Lee Y, Zhang J, Hudak C, Meyer C, Prummer M, Zoffmann S, Truong H, Ebeling M, Kiialainen A, Gerard R, Xia F, Schinzel R, Amrein K, Cowan C Nat Cell Biol, 2015;17(1):57-67. Species: Human Sample Type: Cell Lysate Application: WB Oxidized alpha1-antitrypsin stimulates the release of monocyte chemotactic protein-1 from lung epithelial cells: potential role in emphysema. Authors: Li Z, Alam S, Wang J, Sandstrom CS, Janciauskiene S, Mahadeva R Am. J. Physiol. Lung Cell Mol. Physiol., 2009;297(2):L388-400. Species: Human Sample Type: Cell Lysates Application: WB Type I IFNs play a role in early resistance, but subsequent susceptibility, to the African trypanosomes. Authors: Lopez R, Demick KP, Mansfield JM, Paulnock DM J. Immunol., 2008;181(7):4908-17. Species: Mouse Sample Type: Cell Lysates Application: WB Distinct role of c-Jun N-terminal kinase isoforms in human neutrophil apoptosis regulated by tumor necrosis factor-alpha and granulocyte-macrophage colony-stimulating factor. Authors: Kato T, Noma H, Kitagawa M, Takahashi T, Oshitani N, Kitagawa S J. Interferon Cytokine Res., 2008;28(4):235-43. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human JNK1 Met1-Gln384 Accession # P45983
生物活性	Human, Mouse, Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Members of the MAPK family, the c-Jun N-terminal kinases (JNKs) are activated by environmental stresses and inflammatory cytokines. Ten JNK isoforms are created by alternative splicing of mRNA transcripts derived from three genes: JNK1, JNK2, and JNK3. All JNKs are activated by dual phosphorylation; at T183/Y185 for JNK1 and 2, and T221/Y223 for JNK3. Activated JNKs translocate to the nucleus where they regulate the activity of several transcription factors; including the c-Jun component of AP-1 and ATF-2.
运输条件	Blue Ice
存放说明	4℃
参考文献	Airway Inflammation and Remodeling Apoptosis Intracellular Kinases Cancer Biomarkers Chemokine Receptor Intracellular Signaling C-type Lectin Receptors Diabetic Peripheral Neuropathy EMT Induction GDNF Family ICC / IHC Images: JNK IL-1 Family Interactive Pathway: IL-1 Family Signaling Pathways Intracellular Kinases Intracellular Kinases in the Akt Pathway Intracellular Signaling Molecules in Angiogenesis Neurotrophin/Trk Family NOD-like Receptors and the Inflammasome Positive Regulators of the Jak/STAT Pathway TNF Superfamily Regulators Toll-Like Receptors Wnt Intracellular Signaling

参考图片

Detection of Human/Mouse/Rat JNK by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, U937 human histiocytic lymphoma cell line, C6 rat glioma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat JNK Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1387) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands for JNK were detected at approximately 46 and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of recombinant JNK family members by Western Blot. Western blot shows recombinant human JNK1, JNK2, and JNK3 (1 ng/lane). PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat JNK Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1387) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

JNK in Human Prostate Cancer Tissue. JNK was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Rabbit Anti-Human/Mouse/Rat JNK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1387) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.

JNK in Rat Kidney. JNK was detected in perfusion fixed frozen sections of rat kidney using Rabbit Anti-Human/Mouse/Rat JNK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1387) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.