Human/Mouse/Rat p38 gamma Affinity Purified PAb (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human/Mouse/Rat p38 gamma Affinity Purified PAb (25 UG)

货号： AF1347-SP 基本售价： 1407.8 元规格： -

产品信息

概述
货号	AF1347-SP
别名	EC 2.7.11; ERK3; ERK-6; ERK6MAPK 12; Extracellular signal-regulated kinase 6; MAP kinase 12; MAP kinase p38 gamma; MAPK12; mitogen-activated protein kinase 12; mitogen-activated protein kinase 3; Mitogen-activated protein kinase p38 gamma; p38gamma; PRKM12; SAPK-3; SAPK3EC 2.7.11.24; Stress-activated protein kinase 3
反应种属	Human/Mouse/Rat
应用	Western Blot(0.2 µg/mL) Simple Western(2 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human, mouse, and rat p38 gamma in Western blots. The antibody is highly specific for p38 gamma and in Western blots, shows extremely weak cross-reactivity with recombinant p38 alpha, p38 beta, or p38δ after prolonged exposure to film.
使用方法	Western Blot: 0.2 µg/mL Simple Western: 2 µg/mL Immunohistochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6300 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Kif13b Regulates PNS and CNS Myelination through the Dlg1 Scaffold Authors: R Noseda, M Guerrero-V, V Alberizzi, SC Previtali, DL Sherman, M Palmisano, RL Huganir, KA Nave, A Cuenda, ML Feltri, PJ Brophy, A Bolino PLoS Biol, 2016;14(4):e1002440. Species: Mouse Sample Type: tissue lysates Application: WB p38gamma MAPK Is a Therapeutic Target for Triple-Negative Breast Cancer by Stimulation of Cancer Stem-Like Cell Expansion. Authors: Qi X, Yin N, Ma S, Lepp A, Tang J, Jing W, Johnson B, Dwinell M, Chitambar C, Chen G Stem Cells, 2015;33(9):2738-47. Species: Human Sample Type: Cell Lysates Application: WB Differential tissue expression and activation of p38 MAPK alpha, beta, gamma, and delta isoforms in rheumatoid arthritis. Authors: Korb A, Tohidast-Akrad M, Cetin E, Axmann R, Smolen J, Schett G Arthritis Rheum., 2006;54(9):2745-56. Species: Human Sample Type: Tissue Homogenates Application: IHC
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human p38 gamma Accession # P53778
生物活性	Human, Mouse, Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	p38 mitogen-activated protein kinase (MAPK)gamma, also known as p38 gamma, MAPK12, SAPK3 and ERK6 is a serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays a role in myoblast differentiation and also in the down-regulation of cyclin D1 in response to hypoxia in adrenal cells.
运输条件	Blue Ice
存放说明	4℃
参考文献	Apoptosis Intracellular Kinases Chemokine Receptor Intracellular Signaling C-type Lectin Receptors Diabetic Peripheral Neuropathy GDNF Family IL-1 Family IL-17 Family Intracellular Kinases Intracellular Signaling Molecules in Angiogenesis Neurotrophin/Trk Family NOD-like Receptors and the Inflammasome Technical Notes: phospho-specific ELISAs TNF Superfamily Regulators Toll-Like Receptors VEGF Family

参考图片

Detection of Mouse/Rat p38 gamma by Western Blot. Western blot shows lysates of PC-12 rat adrenal pheochromocytoma cell line, C6 rat glioma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat p38 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1347) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for p38 gamma was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Mouse and Rat p38 gamma by Simple Western^TM. Simple Western lane view shows lysates of C2C12 mouse myoblast cell line and PC‑12 rat adrenal pheochromocytoma cell line, loaded at 0.2 mg/mL. A specific band was detected for p38 gamma at approximately 48 kDa (as indicated) using 2 µg/mL of Rabbit Anti-Human/Mouse/Rat p38 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1347). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of human p38 gamma by Western Blot. Western blot shows recombinant human p38 beta, p38 gamma, p38δ and p38 alpha (1 ng/lane). PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat p38 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1347) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for p38 gamma was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.