| 货号 | AF4785-SP |
| 别名 | ADRBK2; adrenergic, beta, receptor kinase 2; BARK2; BARK2beta-adrenergic receptor kinase 2; Beta-ARK-2; EC 2.7.11; EC 2.7.11.15; G-protein-coupled receptor kinase 3; GRK3; GRK3Beta-ARK-2 | 全称 | G protein-coupled Receptor Kinase 3 |
| 应用 | Western Blot,Simple Western |
| 目标/特异性 | Detects human GRK3 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Simple Western: 20 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 157 (Human); 320129 (Mouse); 25372 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human GRK3 Pro469-Leu688 Accession # P35626 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | G protein-coupled receptor kinases (GRKs) are important modulators of G protein-coupled receptor (GPCR) signaling. Receptor phosphorylation by specific GRKs plays a key role in triggering rapid desensitization. The GRK family consists of 7 isoforms that share a central catalytic domain with homology to other serine/threonine kinases. The catalytic domain is flanked by an amino-terminal RGS domain of 183‑188 amino acids and a carboxyl-terminus of variable length. GRK3 (also known as beta -adrenergic receptor kinase 2, or beta ARK2) and GRK2 ( beta ARK1) are members of the beta -adrenergic receptor kinase subfamily. GRK3 may phosphorylate alpha -synuclein at S129, suggesting a potential involvement for this kinase in Parkinson’s disease. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human GRK3 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and CEM human T-lymphoblastoid cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human GRK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4785) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GRK3 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of Human GRK3 by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and CEM human T-lymphoblastoid cell line, loaded at 0.2 mg/mL. A specific band was detected for GRK3 at approximately 80-81 kDa (as indicated) using 20 µg/mL of Sheep Anti-Human GRK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4785) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
