| 货号 | AF37471-SP |
| 别名 | catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1B; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta | 全称 | Interleukin 1 beta |
| 应用 | CyTOF-ready,Neutralization |
| 目标/特异性 | Detects canine IL‑1 beta /IL‑1F2 beta in direct ELISAs. |
| 使用方法 | CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Neutralization: Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 3-18 ng/mL in the presence of 20 pg/mL Recombinant Canine IL‑1 beta /IL‑1F2. |
| 来源 | Polyclonal Sheep IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant canine IL‑1 beta /IL‑1F2 Asp114-Ser265 Accession # Q28292 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Canine |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 22% amino acid (aa) identity in dog. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL‑1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1 - 4). The canine IL-1 beta cDNA encodes a 266 aa precursor. A 114 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature canine IL-1 beta shares 68-78% aa sequence identity with cotton rat, equine, feline, human, mouse, porcine, rat, and rhesus macaque IL-1 beta. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Cell Proliferation Induced by IL-1 beta /IL-1F2 and Neutralization by Canine IL‑1 beta /IL‑1F2 Antibody. Recombinant Canine IL-1 beta /IL-1F2 (Catalog # 3747-CL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Canine IL-1 beta /IL-1F2 (20 pg/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Canine IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF37471). The ND50 is typically 3‑18 ng/mL. |
