Human Phospho-FAK (Y925) Affinity Purified Polyclonal Ab (25 UG)
货号：
AF44671-SP 基本售价：
1378.1 元 规格：
-
产品信息
概述| 货号 | AF44671-SP |
| 别名 | EC 2.7.10; FADK 1; FADK1; FADKFAK-related non-kinase polypeptide; FAK1FRNK; FAKEC 2.7.10.2; focal adhesion kinase 1; pp125FAK; Protein-tyrosine kinase 2; PTK2 protein tyrosine kinase 2; PTK2 |
全称 | Focal adhesion kinase 1 | | 反应种属 | Human |
| 应用 | Western Blot(0.5 µg/mL)
|
| 目标/特异性 | Detects human FAK when phosphorylated at Y925. |
| 使用方法 | Western Blot: 0.5 µg/mL
|
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 5747 (Human); 14083 (Mouse); 25614 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Phosphopeptide containing human FAK Y925 site |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | Focal adhesion kinase 1 (FAK) is a ubiquitously expressed non-receptor protein tyrosine kinase that is concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. This cellular localization is directed by a "Focal Adhesion Targeting" (FAT) sequence, a 125 amino acid sequence at the C-terminus. FAK plays an important role in migration, cell spreading, differentiation, cytoskeleton protein phosphorylation, apoptosis and acceleration of the G1 to S phase transition of the cell cycle. It associates with several different signaling proteins such as Src-family PTKs, p130Cas, Shc, Grb2, PI 3-kinase, and paxillin. This enables FAK to function within a network of integrin-stimulated signaling pathways leading to the activation of targets such as the ERK and JNK/mitogen-activated protein kinase pathways. FAK is also linked to oncogenes at biochemical and functional levels. Increased expression and/or activity of FAK in various tumors has been correlated with enhanced migration and invasiveness of human tumor cells in addition to promoting increased cell proliferation. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Chemokine Receptor Intracellular Signaling
- Focal Adhesions
- GDNF Family
- ICC / IHC Images: FAK
- Intracellular Kinases in the Akt Pathway
- Intracellular Signaling Molecules in Angiogenesis
- Mesenchymal Cells and EMT
- Repulsive Guidance Molecules and Receptors
- VEGF Family
|
参考图片Detection of Human Phospho-FAK (Y925) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-FAK (Y925) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF44671) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-FAK (Y925) at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
