概述| 货号 | AF5009-SP |
| 别名 | CDHF12MTC1; CDHR16; EC 2.7.10; Hirschsprung disease 1; Hirschsprung disease); MEN2A; MEN2B; multiple endocrine neoplasia and medullary thyroid carcinoma 1; ret proto-oncogene; RET transforming sequence |
全称 | Proto-oncogene Tyrosine-protein Kinase Receptor Ret | | 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL)
|
| 目标/特异性 | Detects human Ret when phosphorylated at Y1062 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL
|
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 5979 (Human); 19713 (Mouse) |
| 应用文献 |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Endoneurial macrophages induce perineural invasion of pancreatic cancer cells by secretion of GDNF and activation of RET tyrosine kinase receptor.
Authors: Cavel O, Shomron O, Shabtay A, Vital J, Trejo-Leider L, Weizman N, Krelin Y, Fong Y, Wong R, Amit M, Gil Z
Cancer Res, 2012;72(22):5733-43.
Species: Human
Sample Type: Cell Lysates
Application: WB
Mortalin (GRP75/HSPA9) upregulation promotes survival and proliferation of medullary thyroid carcinoma cells.
Authors: Starenki D, Hong S, Lloyd R, Park J
Oncogene, 0;34(35):4624-34.
Species: Human
Sample Type: Cell Lysates
Application: WB |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Phosphopeptide containing human Ret Y1062 site |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The GDNF family of neurotrophic factors forms a subfamily within the TGF-beta superfamily. These proteins are potent survival factors for various central and peripheral neurons during development and the adult animal. The GDNF family members (GDNF, neurturin, artemin and persephin) signal through multicomponent receptors that consist of the Ret receptor tyrosine kinase and one of four glycosyl-phosphatidylinositol (GPI)-linked ligand-binding subunits (GFR alpha -1‑4). GFR alpha ‑1 ‑2, ‑3 and ‑4 are the preferred ligand-binding subunits for GDNF, neurturin, artemin and persephin, respectively. The Ret tyrosine-kinase receptor is encoded by the c-ret proto-oncogene. Mutations of the ret gene have been associated with various human diseases affecting tissues derived from the neural crest, including Hirschsprung’s disease, multiple endocrine neoplasia MEN2A and MEN2B, and familial medullary thyroid carcinoma. Human and mouse Ret share 83% amino acid sequence homology (77% homology in the extracellular domain and 93% homology in the cytoplasmic domain). Although Ret does not bind GDNF ligands directly, the extracellular domain of Ret binds the GDNF-GFR-alpha complex with high affinity and is a potent GDNF antagonist in the presence of soluble GFR-alpha (1‑4). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Trupp, M. et al. (1998) Mol. Cell. Neurosci. 11:47.
- Enokido, Y. et al. (1998) Curr. Biol. 8:1019.
- Carlomagno, F. et al. (1998) Endocrinology 139:3613.
- Baloh, R. et al. (1998) Neuron 21:1291.
|
参考图片Detection of Human Phospho-Ret (Y1062) by Western Blot. Western blot shows lysates of TT human medullary thyroid cancer cell line untreated (-) or treated (+) with 100 μM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Human Phospho-Ret (Y1062) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5009), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Ret (Y1062) at approximately 175 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
