Human LAP (TGF-beta 1) Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human LAP (TGF-beta 1) Affinity Purified Polyclonal Ab (25 UG)

货号： AF-246-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-246-SP
别名	LAP (TGFbeta 1); LAP (TGF-beta 1)
全称	Latency-associated Peptide
反应种属	Human
应用	Western Blot,Simple Western,Immunocytochemistry,Neutralization
目标/特异性	Detects human LAP TGF‑ beta 1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody is specific for the LAP derived from the TGF-beta 1 precursor, but does not react with LAP from the TGF-beta 2 precursor.
使用方法	Western Blot: 2 µg/mL Simple Western: 20 µg/mL Immunocytochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize LAP TGF‑ beta 1 inhibition of TGF‑ beta 1 growth inhibition in the HT‑2 mouse T cell line. Tsang, M. et al. (1995) Cytokine 7:389. The Neutralization Dose (ND₅₀) is typically 0.4-2 µg/mL in the presence of 500 ng/mL Recombinant Human LAP TGF‑ beta 1 and 1 ng/mL TGF‑ beta 1.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7040 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Cutting Edge: ERK1 Mediates the Autocrine Positive Feedback Loop of TGF-? and Furin in Glioma-Initiating Cells Authors: E Ventura, M Weller, I Burghardt J. Immunol., 2017;0(0):. Species: Human Sample Type: Cell Lysates Application: WB Secretome and degradome profiling shows that Kallikrein-related peptidases 4, 5, 6, and 7 induce TGFbeta-1 signaling in ovarian cancer cells. Authors: Shahinian H, Loessner D, Biniossek M, Kizhakkedathu J, Clements J, Magdolen V, Schilling O Mol Oncol, 2014;8(1):68-82. Species: Human Sample Type: Cell Culture Supernates Application: WB Identification of bone morphogenetic protein 7 (BMP7) as an instructive factor for human epidermal Langerhans cell differentiation. Authors: Yasmin N, Bauer T, Modak M, Wagner K, Schuster C, Koffel R, Seyerl M, Stockl J, Elbe-Burger A, Graf D, Strobl H J Exp Med, 2013;210(12):2597-610. Species: Human Sample Type: Whole Tissue Application: IHC Frozen TGF-beta mediates homing of bone marrow-derived human mesenchymal stem cells to glioma stem cells. Authors: Shinojima N, Hossain A, Takezaki T, Fueyo J, Gumin J, Gao F, Nwajei F, Marini F, Andreeff M, Kuratsu J, Lang F Cancer Res, 2013;73(7):2333-44. Species: Human Sample Type: Whole Cells Application: Neut Nonmyelinating Schwann cells maintain hematopoietic stem cell hibernation in the bone marrow niche. Authors: Yamazaki S, Ema H, Karlsson G, Yamaguchi T, Miyoshi H, Shioda S, Taketo MM, Karlsson S, Iwama A, Nakauchi H Cell, 2011;147(5):1146-58. Species: Mouse Sample Type: Whole Cells Application: ICC Diverse functions of reactive cysteines facilitate unique biosynthetic processes of aggregate-prone interleukin-31. Authors: Shen M, Siu S, Byrd S, Edelmann KH, Patel N, Ketchem RR, Mehlin C, Arnett HA, Hasegawa H Exp. Cell Res., 2011;317(7):976-93. Species: Human Sample Type: Cell Lysates Application: WB Human pregnancy specific beta-1-glycoprotein 1 (PSG1) has a potential role in placental vascular morphogenesis. Authors: Ha CT, Wu JA, Irmak S, Lisboa FA, Dizon AM, Warren JW, Ergun S, Dveksler GS Biol. Reprod., 2010;83(1):27-35. Species: Human Sample Type: Whole Cells Application: Neut Transforming growth factor-beta signaling in hypertensive remodeling of porcine aorta. Authors: Popovic N, Bridenbaugh EA, Neiger JD, Hu JJ, Vannucci M, Mo Q, Trzeciakowski J, Miller MW, Fossum TW, Humphrey JD, Wilson E Am. J. Physiol. Heart Circ. Physiol., 2009;297(6):H2044-53. Species: Porcine Sample Type: Whole Tissue Application: IHC Induction of epithelial-mesenchymal transition in primary airway epithelial cells from patients with asthma by transforming growth factor-beta1. Authors: Hackett TL, Warner SM, Stefanowicz D, Shaheen F, Pechkovsky DV, Murray LA, Argentieri R, Kicic A, Stick SM, Bai TR, Knight DA Am. J. Respir. Crit. Care Med., 2009;180(2):122-33. Species: Human Sample Type: Whole Cells Application: Neut Transglutaminase 2 limits murine peritoneal acute gout-like inflammation by regulating macrophage clearance of apoptotic neutrophils. Authors: Rose DM, Sydlaske AD, Agha-Babakhani A, Johnson K, Terkeltaub R Arthritis Rheum., 2006;54(10):3363-71. Species: Mouse Sample Type: Whole Cells Application: Neut Compromised lymphocytes infiltrate hepatocellular carcinoma: the role of T-regulatory cells. Authors: Unitt E, Rushbrook SM, Marshall A, Davies S, Gibbs P, Morris LS, Coleman N, Alexander GJ Hepatology, 2005;41(4):722-30. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function. Authors: Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB Blood, 2005;105(2):750-8. Species: Human Sample Type: Cell Culture Supernates Application: Neut
纯化方式	Antigen Affinity-purified
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived and Chinese hamster ovary cell line CHO-derived recombinant human LAP TGF‑ beta 1 Leu30-Ser390 Accession # P01137
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	TGF-beta 1 (transforming growth factor beta 1) and the closely related TGF-beta 2 and -beta 3 are members of the large TGF-beta superfamily. TGF‑ beta proteins are highly pleiotropic cytokines that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1‑3). Human TGF-beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein (4). A furin-like convertase processes the proprotein within the trans-Golgi to generate an N‑terminal 249 aa (aa 30-278) latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF-beta 1 (aa 279-390) (4‑6). Disulfide-linked homodimers of LAP and TGF-beta 1 remain non‑covalently associated after secretion, forming the small latent TGF-beta 1 complex (4‑8). Purified LAP is also capable of associating with active TGF-beta with high affinity, and can neutralize TGF-beta activity (9). Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (5‑7). TGF-beta activation from latency is controlled both spatially and temporally, by multiple pathways that include actions of proteases such as plasmin and MMP9, and/or by thrombospondin 1 or selected integrins (5, 8). The LAP portion of human TGF-beta 1 shares 91%, 92%, 85%, 86% and 88% aa identity with porcine, canine, mouse, rat and equine TGF-beta 1 LAP, respectively, while mature human TGF-beta 1 portion shares 100% aa identity with porcine, canine and bovine TGF-beta 1, and 99% aa identity with mouse, rat and equine TGF-beta 1. Although different isoforms of TGF-beta are naturally associated with their own distinct LAPs, the TGF-beta 1 LAP is capable of complexing with, and inactivating, all other human TGF-beta isoforms and those of most other species (9). Mutations within the LAP are associated with Camurati-Engelmann disease, a rare sclerosing bone dysplasia characterized by inappropriate presence of active TGF-beta 1 (10).
运输条件	Blue Ice
存放说明	4℃
参考文献	Dunker, N. & K. Krieglstein (2000) Eur. J. Biochem. 267:6982. Wahl, S.M. (2006) Immunol. Rev. 213:213. Chang, H. et al. (2002) Endocr. Rev. 23:787. Derynck, R. et al. (1985) Nature 316:701. Dabovic, B. and D.B. Rifkin (2008) “TGF-beta Bioavailability” in The TGF-beta Family. Derynck, R. and K. Miyazono (eds): Cold Spring Harbor Laboratory Press, p. 179. Brunner, A.M. et al. (1989) J. Biol. Chem. 264:13660. Miyazono, K. et al. (1991) EMBO J. 10:1091. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601. Miller, D.M. et al. (1992) Mol. Endocrinol. 6:694. Janssens, K. et al. (2003) J. Biol. Chem. 278:7718.

参考图片

Detection of Human LAP (TGF-beta 1) by Western Blot. Western blot shows lysates of Saos‑2 human osteosarcoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human LAP (TGF-beta 1) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-246-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for LAP (TGF-beta 1) at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human LAP (TGF-beta 1) by Simple Western^TM. Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line and A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for LAP (TGF-beta 1) at approximately 61 kDa (as indicated) using 20 µg/mL of Goat Anti-Human LAP (TGF-beta 1) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-246-NA) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.

LAP TGF‑ beta 1 Inhibition of TGF‑ beta 1 Activity and Neutralization by Human LAP TGF‑ beta 1 Antibody. Recombinant Human LAP TGF‑ beta 1 (Catalog # 246‑LP) inhibits Recombinant Human TGF‑ beta 1 (Catalog # 240‑B) growth inhibition activity in the HT‑2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human TGF‑ beta 1 (1 ng/mL) activity elicited by Recombinant Human LAP TGF‑ beta 1 (500 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human LAP TGF‑ beta 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-246-NA). The ND₅₀ is typically 0.4‑2 µg/mL.