Human Phospho-Chk2 (T68) Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human Phospho-Chk2 (T68) Affinity Purified Polyclonal Ab (25 UG)

货号： AF1626-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF1626-SP
别名	CDS1; CHEK2; CHK2 checkpoint homolog (S. pombe); EC 2.7.11; EC 2.7.11.1; HuCds1; LFS2; PP1425; Rad53; S.pombe) homolog
全称	Checkpoint Kinase 2
反应种属	Human
应用	Western Blot(0.5 µg/mL) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Reconstitute at 0.2 mg/mL in sterile PBS.
使用方法	Western Blot: 0.5 µg/mL Immunocytochemistry: 5-15 µg/mL
来源	Polyclonal Rabbit IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	11200 (Human); 50883 (Mouse); 114212 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Starvation-induced activation of ATM/Chk2/p53 signaling sensitizes cancer cells to cisplatin. Authors: Shi Y, Felley-Bosco E, Marti T, Orlowski K, Pruschy M, Stahel R BMC Cancer, 2012;12(0):571. Species: Human Sample Type: Cell Lysates Application: WB Multiple DNA damage signaling and repair pathways deregulated by simian virus 40 large T antigen. Authors: Boichuk S, Hu L, Hein J J. Virol., 2010;84(16):8007-20. Species: Human Sample Type: Whole Cells Application: WB Simian virus 40 large T antigen disrupts genome integrity and activates a DNA damage response via Bub1 binding. Authors: Hein J, Boichuk S, Wu J, Cheng Y, Freire R, Jat PS, Roberts TM, Gjoerup OV J. Virol., 2009;83(1):117-27. Species: Human Sample Type: Whole Cells Application: WB RAD51C facilitates checkpoint signaling by promoting CHK2 phosphorylation. Authors: Badie S, Liao C, Thanasoula M, Barber P, Hill MA, Tarsounas M J. Cell Biol., 2009;185(4):587-600. Species: Human Sample Type: Cell Lysates Application: WB Sonic Hedgehog signaling impairs ionizing radiation-induced checkpoint activation and induces genomic instability. Authors: Leonard JM, Ye H, Wetmore C, Karnitz LM J. Cell Biol., 2008;183(3):385-91. Species: Human Sample Type: Cell Lysates Application: WB RNF8-dependent and RNF8-independent regulation of 53BP1 in response to DNA damage. Authors: Sakasai R, Tibbetts R J. Biol. Chem., 2008;283(20):13549-55. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Phosphopeptide containing human Chk2 T68 site
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	ATM phosphorylates the Checkpoint kinase on T68 in response to genotoxic insults. Phosphorylation of T68 promotes the formation of Chk2 oligomers. Oligomerization is a critical event in the activation of the Chk2 kinase as it is required for phosphorylation of additional Chk2 residues that lead to full activation and signaling to downstream effectors.
运输条件	Blue Ice
存放说明	4℃
参考文献	Checkpoint DNA Damage and Repair Intracellular Kinases in the Akt Pathway

参考图片

Detection of Human Phospho-Chk2 (T68) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 1 hour. PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human Phospho‑Chk2 (T68) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1626) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-Chk2 (T68) was detected at approximately 64 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda -phosphatase ( lambda -PPase) for 60 minutes. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Phospho-Chk2 (T68) in HepG2 Human Cell Line. Phopsho-Chk2 (T68) was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line treated with 1 µM camptothecin using Rabbit Anti-Human Phospho-Chk2 (T68) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1626) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei in treated cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.