Phospho-p27/Kip1 (T198) Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Phospho-p27/Kip1 (T198) Affinity Purified Polyclonal Ab (25 UG)

货号： AF3994-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF3994-SP
别名	CDKN1B; CDKN4; cyclin-dependent kinase inhibitor 1B (p27, Kip1); cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; Kip1; KIP1P27KIP1; MEN1B; MEN4; p27Kip1
全称	p27 Cyclin Dependent Kinase 4 Inhibitor 1B
应用	Western Blot(0.5 µg/mL)
目标/特异性	Detects human p27/Kip1 when phosphorylated at T198. Does not recognize p27/Kip1 when unphosphorylated at T198.
使用方法	Western Blot: 0.5 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1027 (Human); 12576 (Mouse); 83571 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Cytoplasmic p27 promotes epithelial-mesenchymal transition and tumor metastasis via STAT3-mediated Twist1 upregulation. Authors: Zhao D, Besser A, Wander S, Sun J, Zhou W, Wang B, Ince T, Durante M, Guo W, Mills G, Theodorescu D, Slingerland J Oncogene, 2015;34(43):5447-59. Species: Human Sample Type: Cell Lysates Application: IP Cdc6 protein activates p27KIP1-bound Cdk2 protein only after the bound p27 protein undergoes C-terminal phosphorylation. Authors: Uranbileg B, Yamamoto H, Park JH J. Biol. Chem., 2012;287(9):6275-83. Species: Rat Sample Type: Cell Lysates Application: WB A novel p38-MAPK signaling axis modulates neutrophil biology in head and neck cancer. Authors: Dumitru CA, Fechner MK, Hoffmann TK J. Leukoc. Biol., 2012;91(4):591-8. Species: Human Sample Type: Cell Lysates Application: WB Promotion of cytoplasmic mislocalization of p27 by Helicobacter pylori in gastric cancer. Authors: Wen S, So Y, Singh K Oncogene, 2012;31(14):1771-80. Species: Human Sample Type: Cell Lysates Application: WB Homeodomain-interacting Protein Kinase-2 Stabilizes p27kip1 by Its Phosphorylation at Serine 10 and Contributes to Cell Motility. Authors: Pierantoni GM, Esposito F, Tornincasa M, Rinaldo C, Viglietto G, Soddu S, Fusco A J. Biol. Chem., 2011;286(33):29005-13. Species: Human Sample Type: Cell Lysates Application: WB Ablation of Akt2 induces autophagy through cell cycle arrest, the downregulation of p70S6K, and the deregulation of mitochondria in MDA-MB231 cells. Authors: Santi SA, Lee H PLoS ONE, 2011;6(1):e14614. Species: Human Sample Type: Cell Lysates Application: WB The microbiome and butyrate regulate energy metabolism and autophagy in the mammalian colon. Authors: Donohoe DR, Garge N, Zhang X, Sun W, OConnell TM, Bunger MK, Bultman SJ Cell Metab., 2011;13(5):517-26. Species: Mouse Sample Type: Cell Lysates Application: WB Cyclin D1 regulates p27(Kip1) stability in B cells. Authors: Bustany S, Tchakarska G, Sola B Cell. Signal., 2011;23(1):171-9. Species: Mouse Sample Type: Cell Lysates Application: WB Cytoplasmic sequestration of p27 via AKT phosphorylation in renal cell carcinoma. Authors: Kim J, Jonasch E, Alexander A, Short JD, Cai S, Wen S, Tsavachidou D, Tamboli P, Czerniak BA, Do KA, Wu KJ, Marlow LA, Wood CG, Copland JA, Walker CL Clin. Cancer Res., 2009;15(1):81-90. Species: Human Sample Type: Tissue Homogenates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Phosphopeptide containing human p27/Kip1 T198 site
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	The cyclin-dependent kinase (cdk) inhibitor p27/Kip1 is a tumor suppressor protein that acts in the nucleus to enforce cell cycle checkpoints. p27/Kip1 binds to and inhibits many cyclin/cdk complexes, including cyclin D/cdk4, cyclin E/cdk2, and cyclin A/cdk2. This inhibition can subsequently block progression through different phases of the cell cycle. p27/Kip1 is phosphorylated at T198 in cells treated with the AMP-dependent protein kinase (AMPK) activator AICAR (5-amino-4‑imidazolecarboxamide riboside) or glucose withdrawal.
运输条件	Blue Ice
存放说明	4℃
参考文献	Autophagy Cancer Biomarkers Cell Cycle Checkpoint ICC / IHC Images: p27/Kip1 p27/Kip1 IHC Detection Kit

参考图片

Detection of Human Phospho-p27/Kip1 (T198) by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, non-transfected, transfected with plasmid expressing YFP-p27/Kip1 wild type (WT), or transfected with YFP-p27/Kip1 T198A mutant (T198A). PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human Phospho-p27/Kip1 (T198) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3994) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for p27/Kip1 was detected at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.