Phospho-p27/Kip1 (T157) Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Phospho-p27/Kip1 (T157) Affinity Purified Polyclonal Ab (25 UG)

货号： AF1555-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF1555-SP
别名	CDKN1B; CDKN4; cyclin-dependent kinase inhibitor 1B (p27, Kip1); cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; Kip1; KIP1P27KIP1; MEN1B; MEN4; p27Kip1
全称	p27 Cyclin Dependent Kinase 4 Inhibitor 1B
应用	Western Blot(0.5 µg/mL)
目标/特异性	Reconstitute at 0.2 mg/mL in sterile PBS.
使用方法	Western Blot: 0.5 µg/mL
来源	Polyclonal Rabbit IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1027 (Human); 12576 (Mouse); 83571 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Cytoplasmic p27 promotes epithelial-mesenchymal transition and tumor metastasis via STAT3-mediated Twist1 upregulation. Authors: Zhao D, Besser A, Wander S, Sun J, Zhou W, Wang B, Ince T, Durante M, Guo W, Mills G, Theodorescu D, Slingerland J Oncogene, 2015;34(43):5447-59. Species: Human Sample Type: Cell Lysates Application: IP Promotion of cytoplasmic mislocalization of p27 by Helicobacter pylori in gastric cancer. Authors: Wen S, So Y, Singh K Oncogene, 2012;31(14):1771-80. Species: Human Sample Type: Cell Lysates Application: WB XB130 mediates cancer cell proliferation and survival through multiple signaling events downstream of Akt. Authors: Shiozaki A, Shen-Tu G, Bai X, Iitaka D, De Falco V, Santoro M, Keshavjee S, Liu M PLoS ONE, 2012;7(8):e43646. Species: Human Sample Type: Cell Lysates Application: WB Akt isoform-specific inhibition of MDA-MB-231 cell proliferation. Authors: Yang W, Ju JH, Lee KM, Shin I Cell. Signal., 2011;23(1):19-26. Species: Human Sample Type: Cell Lysates Application: WB Homeodomain-interacting Protein Kinase-2 Stabilizes p27kip1 by Its Phosphorylation at Serine 10 and Contributes to Cell Motility. Authors: Pierantoni GM, Esposito F, Tornincasa M, Rinaldo C, Viglietto G, Soddu S, Fusco A J. Biol. Chem., 2011;286(33):29005-13. Species: Human Sample Type: Cell Lysates Application: WB Ablation of Akt2 induces autophagy through cell cycle arrest, the downregulation of p70S6K, and the deregulation of mitochondria in MDA-MB231 cells. Authors: Santi SA, Lee H PLoS ONE, 2011;6(1):e14614. Species: Human Sample Type: Cell Lysates Application: WB Knockdown of protein tyrosine phosphatase SHP-1 inhibits G1/S progression in prostate cancer cells through the regulation of components of the cell-cycle machinery. Authors: Rodriguez-Ubreva FJ, Cariaga-Martinez AE, Cortes MA, Romero-De Pablos M, Ropero S, Lopez-Ruiz P, Colas B Oncogene, 2010;29(3):345-55. Species: Human Sample Type: Whole Cells Application: WB Cytoplasmic sequestration of p27 via AKT phosphorylation in renal cell carcinoma. Authors: Kim J, Jonasch E, Alexander A, Short JD, Cai S, Wen S, Tsavachidou D, Tamboli P, Czerniak BA, Do KA, Wu KJ, Marlow LA, Wood CG, Copland JA, Walker CL Clin. Cancer Res., 2009;15(1):81-90. Species: Human Sample Type: Tissue Homogenates Application: WB Overcoming trastuzumab resistance in HER2-overexpressing breast cancer cells by using a novel celecoxib-derived phosphoinositide-dependent kinase-1 inhibitor. Authors: Tseng PH, Wang YC, Weng SC, Weng JR, Chen CS, Brueggemeier RW, Shapiro CL, Chen CY, Dunn SE, Pollak M, Chen CS Mol. Pharmacol., 2006;70(5):1534-41. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Phosphopeptide containing human p27/Kip1 T157 site
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	The cyclin-dependent kinase (cdk) inhibitor p27/Kip1 is a tumor suppressor protein that acts in the nucleus to enforce cell cycle checkpoints. The Akt protein kinase phosphorylates T157, an amino acid residue that lies within a nuclear localization signal for p27, to prevent nuclear localization and thus preclude the growth inhibitory function of p27.
运输条件	Blue Ice
存放说明	4℃
参考文献	Autophagy Cancer Biomarkers Cell Cycle Checkpoint ICC / IHC Images: p27/Kip1 p27/Kip1 IHC Detection Kit

参考图片

Detection of Human Phospho-p27/Kip1 (T157) by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF-I (Catalog # 291‑G1) for 20 minutes. PVDF membrane was probed with 0.5 µg/mL Human Phospho-p27/Kip1 (T157) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1555) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-p27/Kip1 (T157) was detected at approximately 27 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda -phosphatase ( lambda -PPase) for 1 hour. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.