| 货号 | AF2838-SP |
| 别名 | BZS; EC 3.1.3.16,10q23del; EC 3.1.3.48; EC 3.1.3.67; GLM2; MMAC1 phosphatase and tensin homolog deleted on chromosome 10; MMAC1; MMAC1MGC11227; Mutated in multiple advanced cancers 1; phosphatase and tensin homologDEC; phosphatidylinositol-34,5-trisphosphate 3-phosphatase and dual-specificityprotein phosphatase PTEN; PTEN1; TEP1MHAM | 全称 | Phosphatase and Tensin Homolog Deleted on Chromosome 10 |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human, mouse, and rat PTEN when phosphorylated at S380 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5728 (Human); 19211 (Mouse); 50557 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Blockade of Axl signaling ameliorates HPV16E6-mediated tumorigenecity of cervical cancer | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Phosphopeptide containing human PTEN S380 site |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The tumor suppressor gene PTEN (phosphatase and tensin homolog deleted on chromosome 10), also known as MMAC1 (mutated in multiple advanced cancers 1), encodes a phosphatase that contains the catalytic signature motif (HCXXGXXRS/T) found in all members of the protein tyrosine phosphatase family. In vitro, the recombinant PTEN has both lipid phosphatase and protein phosphatase activities (1, 2). Interestingly, accumulating evidence has shown that the tumor suppressor activity of PTEN relies on its ability to dephosphorylate phosphatidylinositol (3, 4, 5)-triphosphate specifically at position 3 of the inositol ring (3). This activity reduces the levels of phosphatidylinositol (3, 4, 5)-triphosphate which is specifically produced from phosphatidylinositol (4, 5)-diphosphate by PI 3-kinase upon activation by a variety of stimuli. Therefore, PTEN antagonizes PI 3-kinase-induced downstream signaling events and cellular processes including cell growth, apoptosis and cell motility. In vivo, the importance of PTEN catalytic activity in its tumor suppressor functions is underscored by the fact that the majority of PTEN missense mutations detected in tumor specimens target the phosphatase domain and cause a loss in PTEN phosphatase activity (4). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human, Mouse, and Rat Phospho-PTEN (S380) by Western Blot. Western blot shows Human/Mouse/Rat PTEN Monoclonal Antibody (Catalog #MAB847) immunopreciptate of MRC‑5 human embryonic lung fibroblast cell line, mouse and rat brain tissue untreated (-) or treated (+) with 300 U/mL CIP for 1 hour. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse/ |
