Phospho-Chk1 (S345) Affinity Purified Polyclonal Ab (25 UG)
货号：
AF2475-SP 基本售价：
1378.1 元 规格：
-
产品信息
概述| 货号 | AF2475-SP |
| 别名 | Checkpoint, S. pombe, homolog of, 1; CHEK1; CHK1 (checkpoint, S.pombe) homolog; CHK1 checkpoint homolog (S. pombe); CHK1serine/threonine-protein kinase Chk1; EC 2.7.11; EC 2.7.11.1 |
全称 | Checkpoint Kinase 1 | | 应用 | Western Blot(1 µg/mL)
|
| 目标/特异性 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 使用方法 | Western Blot: 1 µg/mL
|
| 来源 | Polyclonal Rabbit IgG |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 1111 (Human); 12649 (Mouse); 140583 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Phosphopeptide containing human Chk1 S345 site |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The Chk1 checkpoint kinase is an integral member of a signaling cascade that controls cell cycle progression. In response to genotoxic or replicative stress, Chk1 is phosphorylated by the ATM or ATM-related kinase (ATR) at S345. In turn, Chk1 phosphorylates downstream effectors, such as p53 or the Cdc25 phosphatases to halt cell cycle progression and allow time for repair of incurred damage. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Checkpoint
- DNA Damage and Repair
- Intracellular Kinases in the Akt Pathway
|
参考图片Detection of Human Phospho-Chk1 (S345) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line exposed to 50 J/m 2 UV-C with a 2 hour recovery. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human Phospho-Chk1 (S345) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2475) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-Chk1 (S345) was detected at approximately 55-60 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda -phosphatase ( lambda -PPase) for 1 hour. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
