Phospho-Chk1 (S317) Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Phospho-Chk1 (S317) Affinity Purified Polyclonal Ab (25 UG)

货号： AF2054-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF2054-SP
别名	Checkpoint, S. pombe, homolog of, 1; CHEK1; CHK1 (checkpoint, S.pombe) homolog; CHK1 checkpoint homolog (S. pombe); CHK1serine/threonine-protein kinase Chk1; EC 2.7.11; EC 2.7.11.1
全称	Checkpoint Kinase 1
应用	Western Blot(1 µg/mL) Simple Western(10 µg/mL)
目标/特异性	Reconstitute at 0.2 mg/mL in sterile PBS.
使用方法	Western Blot: 1 µg/mL Simple Western: 10 µg/mL
来源	Polyclonal Rabbit IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1111 (Human); 12649 (Mouse); 140583 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Dbf4 is direct downstream target of ataxia telangiectasia mutated (ATM) and ataxia telangiectasia and Rad3-related (ATR) protein to regulate intra-S-phase checkpoint. Authors: Lee AY, Chiba T, Truong LN J. Biol. Chem., 2012;287(4):2531-43. Species: Human Sample Type: Cell Lysates Application: WB Poly(ADP-Ribose) polymerase inhibition synergizes with 5-fluorodeoxyuridine but not 5-fluorouracil in ovarian cancer cells. Authors: Huehls AM, Wagner JM, Huntoon CJ, Geng L, Erlichman C, Patel AG, Kaufmann SH, Karnitz LM Cancer Res., 2011;71(14):4944-54. Species: Human Sample Type: Cell Lysates Application: WB Functional connection between Rad51 and PML in homology-directed repair. Authors: Boichuk S, Hu L, Makielski K, Pandolfi PP, Gjoerup OV PLoS ONE, 2011;6(10):e25814. Species: Human Sample Type: Cell Lysates Application: WB Multiple DNA damage signaling and repair pathways deregulated by simian virus 40 large T antigen. Authors: Boichuk S, Hu L, Hein J J. Virol., 2010;84(16):8007-20. Species: Human Sample Type: Cell Lysates Application: WB Simian virus 40 large T antigen disrupts genome integrity and activates a DNA damage response via Bub1 binding. Authors: Hein J, Boichuk S, Wu J, Cheng Y, Freire R, Jat PS, Roberts TM, Gjoerup OV J. Virol., 2009;83(1):117-27. Species: Human Sample Type: Whole Cells Application: WB RNF8-dependent and RNF8-independent regulation of 53BP1 in response to DNA damage. Authors: Sakasai R, Tibbetts R J. Biol. Chem., 2008;283(20):13549-55. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Phosphopeptide containing human Chk1 S317 site
生物活性	Human, Mouse, Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	The Chk1 checkpoint kinase is an integral member of a signaling cascade that controls cell cycle progression. In response to genotoxic or replicative stress, Chk1 is phosphorylated by ATM or ATM-related kinases (ATR) at S317. In turn, Chk1 phosphorylates downstream effectors, such as p53 or the Cdc25 phosphatases to halt cell cycle progression and allow time for repair of incurred damage.
运输条件	Blue Ice
存放说明	4℃
参考文献	Checkpoint DNA Damage and Repair Intracellular Kinases in the Akt Pathway

参考图片

Detection of Human Phospho-Chk1 (S317) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or exposed (+) to 50 J/m² UV-C for the indicated time. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human/Mouse/Rat Phospho-Chk1 (S317) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2054) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-Chk1 (S317) was detected at approximately 56 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda -phosphatase ( lambda -PPase) for 1 hour. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Phospho-Chk1 (S317) by Simple Western^TM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 50 J/m² ultraviolet light (UV) for 2 hours, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Chk1 (S317) at approximately 60 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Chk1 (S317) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2054). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.