Phospho-ATM (S1981) Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Phospho-ATM (S1981) Affinity Purified Polyclonal Ab (25 UG)

货号： AF1655-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF1655-SP
别名	AT mutated; A-T mutated; AT1; ATA; ataxia telangiectasia mutated (includes complementation groups A, C and D); ataxia telangiectasia mutatedATD; ATC; ATDC; ATE; DKFZp781A0353; EC 2.7.11.1; MGC74674; serine-protein kinase ATM; TEL1; TEL1, telomere maintenance 1, homolog; TELO1; TPLL
全称	Ataxia Telangiectasia-mutated
应用	Western Blot(1 µg/mL) Simple Western(10 µg/mL)
目标/特异性	Reconstitute at 0.2 mg/mL in sterile PBS.
使用方法	Western Blot: 1 µg/mL Simple Western: 10 µg/mL
来源	Polyclonal Rabbit IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	472 (Human); 11920 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. DNA-PK-A candidate driver of hepatocarcinogenesis and tissue biomarker that predicts response to treatment and survival. Authors: Cornell L, Munck J, Alsinet C, Villanueva A, Ogle L, Willoughby C, Televantou D, Thomas H, Jackson J, Burt A, Newell D, Rose J, Manas D, Shapiro G, Curtin N, Reeves H Clin Cancer Res, 2015;21(4):925-33. Species: Human Sample Type: Whole Tissue Application: IHC - paraffin Severe reaction to radiotherapy for breast cancer as the presenting feature of ataxia telangiectasia. Authors: Byrd PJ, Srinivasan V, Last JI Br. J. Cancer, 2012;106(2):262-8. Species: Human Sample Type: Cell Lysates Application: WB Lymphoid tumours and breast cancer in ataxia telangiectasia; substantial protective effect of residual ATM kinase activity against childhood tumours. Authors: Reiman A, Srinivasan V, Barone G Br. J. Cancer, 2011;105(4):586-91. Species: Human Sample Type: Cell Lysates Application: WB RAD51D protects against MLH1-dependent cytotoxic responses to O(6)-methylguanine. Authors: Rajesh P, Rajesh C, Wyatt MD DNA Repair (Amst.), 2010;9(4):458-67. Species: Mouse Sample Type: Cell Lysates Application: WB Inhibition of CHK1 kinase by Go6976 converts 8-chloro-adenosine-induced G2/M arrest into S arrest in human myelocytic leukemia K562 cells. Authors: Jia XZ, Yang SY, Zhou J, Li SY, Ni JH, An GS, Jia HT Biochem. Pharmacol., 2009;77(5):770-80. Species: Human Sample Type: Cell Lysates Application: WB Sonic Hedgehog signaling impairs ionizing radiation-induced checkpoint activation and induces genomic instability. Authors: Leonard JM, Ye H, Wetmore C, Karnitz LM J. Cell Biol., 2008;183(3):385-91. Species: Mouse Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Phosphopeptide containing human ATM S1981 site
生物活性	Human, Mouse, Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	The Ataxia telangiectasia-mutated (ATM) protein kinase exists as a dimer in the cell nucleus. Changes in DNA structure induced by genotoxic stress lead to activation of ATM and phosphorylation of S1981 in trans. Once S1981 is phosphorylated, the dimer dissociates and active ATM monomers signal to downstream targets.
运输条件	Blue Ice
存放说明	4℃
参考文献	Checkpoint DNA Damage and Repair ICC / IHC Images: ATM Intracellular Kinases in the Akt Pathway p53 Pathway

参考图片

Detection of Human/Mouse/Rat Phospho-ATM (S1981) by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 1 hour. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human/Mouse/Rat Phospho-ATM (S1981) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1655) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-ATM (S1981) was detected at approximately 370 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda ‑phosphatase ( lambda -PPase) for 1 hour. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Phospho-ATM (S1981) by Simple Western^TM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 µM Camptothecin (CPT) for 1 hour, loaded at 0.2 mg/mL. A specific band was detected for Phospho-ATM (S1981) at approximately 270 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-ATM (S1981) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1655). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.