Mouse Oncostatin M (OSM) Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse Oncostatin M (OSM) Affinity Purified Polyclonal Ab (25 UG)

货号： AF-495-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-495-SP
别名	MGC20461; oncostatin M; oncostatin-M; OSM
反应种属	Mouse
应用	Western Blot,Immunohistochemistry,Neutralization
目标/特异性	Detects mouse OSM in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant human OSM is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize Oncostatin M/OSM-induced proliferation in the NIH‑3T3 mouse embryonic fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 0.6-3.0 µg/mL in the presence of 15 ng/mL Recombinant Mouse Oncostatin M/OSM.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5008 (Human); 18413 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Anti-OSM Antibody Inhibits Tubulointerstitial Lesion in a Murine Model of Lupus Nephritis Authors: Q Liu, Y Du, K Li, W Zhang, X Feng, J Hao, H Li, S Liu Mediators Inflamm., 2017;2017(0):3038514. Species: Mouse Sample Type: In Vivo Application: Neut Oncostatin M overexpression induces skin inflammation but is not required in the mouse model of imiquimod-induced psoriasis-like inflammation Eur J Immunol, 2016;0(0):. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Oncostatin m, an inflammatory cytokine produced by macrophages, supports intramembranous bone healing in a mouse model of tibia injury. Authors: Guihard P, Boutet M, Brounais-Le Royer B, Gamblin A, Amiaud J, Renaud A, Berreur M, Redini F, Heymann D, Layrolle P, Blanchard F Am J Pathol, 2015;185(3):765-75. Species: Mouse Sample Type: Whole Tissue Application: IHC Not Specified Activation of NFAT signaling establishes a tumorigenic microenvironment through cell autonomous and non-cell autonomous mechanisms. Authors: Tripathi, P, Wang, Y, Coussens, M, Manda, K R, Casey, A M, Lin, C, Poyo, E, Pfeifer, J D, Basappa, N, Bates, C M, Ma, L, Zhang, H, Pan, M, Ding, L, Chen, F Oncogene, 2014;33(14):1840-9. Oncostatin M acting via OSMR, augments the actions of IL-1 and TNF in synovial fibroblasts. Authors: Le Goff B, Singbrant S, Tonkin B, Martin T, Romas E, Sims N, Walsh N Cytokine, 2014;68(2):101-9. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Long term oncostatin M treatment induces an osteocyte-like differentiation on osteosarcoma and calvaria cells. Authors: Brounais B, David E, Chipoy C, Trichet V, Ferre V, Charrier C, Duplomb L, Berreur M, Redini F, Heymann D, Blanchard F Bone, 2009;44(5):830-9. Species: Mouse Sample Type: Cell Lysates Application: Neut Cardiotrophin-1 in choroid plexus and the cerebrospinal fluid circulatory system. Authors: Gard AL, Gavin E, Solodushko V, Pennica D Neuroscience, 2004;127(1):43-52. Species: Mouse Sample Type: Tissue Homogenates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse OSM Ala24-Arg206 Accession # P53347
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Oncostatin M (OSM) is a member of a cytokine subfamily that includes IL-6, IL-11, LIF, CNTF, and cardiotrophin-1. These cytokines have overlapping biological functions and shared receptor components. Mouse OSM was cloned and identified as an immediate early gene induced in various myeloid and lymphoid cell lines by a subset of cytokines including IL-2, IL-3, GM-CSF, and EPO. The mouse OSM cDNA encodes a 263 amino acid residue precursor protein that shows 48% identity with human OSM. Similar to human OSM, the C-terminal region of mouse OSM contains a highly charged region. Deletion of this C-terminal region appears to be essential for the formation of biologically active mOSM. The biological activity of human OSM has been shown to be mediated either by the LIF/OSM receptor complex composed of gp130 and LIF R alpha or by a human OSM specific receptor composed of gp130 and OSM R alpha. It remains to be determined if the biological activities of mouse OSM can also be mediated by both receptor complexes in mouse cells.
运输条件	Blue Ice
存放说明	4℃
参考文献	Yoshimura, A. et al. (1996) The EMBO Journal 15:1055. Ray, P. et al. (1996) Endocrinology 137:1151. Rose, T.M. and A.G. Bruce (1994) in Guidebook to Cytokines and Their Receptors, N.A. Nicola, editor, Oxford University Press, New York, p. 127.

参考图片

Oncostatin M/OSM in Mouse Embryo. Oncostatin M/OSM was detected in immersion fixed frozen sections of mouse embryo using Mouse Oncostatin M/OSM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-495-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Oncostatin M/OSM in Mouse Embryo. Oncostatin M/OSM was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c, section through spinal cord) using Mouse Oncostatin M/OSM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-495-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Cell Proliferation Induced by Oncostatin M/OSM and Neutralization by Mouse Oncostatin M/OSM Antibody. Recombinant Mouse Oncostatin M/OSM (Catalog # 495-MO) stimulates proliferation in the NIH‑3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Oncostatin M/OSM (15 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Oncostatin M/OSM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-495-NA). The ND₅₀ is typically 0.6-3.0 µg/mL.