Mouse Osteopontin (OPN) Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Mouse Osteopontin (OPN) Affinity Purified Polyclonal Ab (25 UG)

货号： AF808-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF808-SP
别名	BNSP; Bone sialoprotein 1; Eta-1; MGC110940; Nephropontin; OPN; osteopontin; secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1); secreted phosphoprotein-1 (osteopontin, bone sialoprotein); Spp1; SPP-1; SPP1/CALPHA1 fusion; Urinary stone protein; uropontin
全称	Secreted Phosphoprotein 1 [BNSP]
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) ELISA Capture (Matched Antibody Pair)(0.2-0.8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( )
目标/特异性	Detects mouse Osteopontin (OPN) in ELISAs and Western blots. In sandwich ELISAs, less than 3% cross-reactivity with recombinant human OPN is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL ELISA Capture (Matched Antibody Pair): 0.2-0.8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize Osteopontin/OPN-mediated adhesion of the HEK293 human embryonic kidney cell line. The Neutralization Dose (ND₅₀) is typically 1-3 µg/mL in the presence of 2 µg/mL Recombinant Mouse Osteopontin/OPN.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6696 (Human); 20750 (Mouse); 25353 (Rat); 281499 (Bovine)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The P2X4 receptor is required for neuroprotection via ischemic preconditioning Sci Rep, 2016;6(0):25893. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Selective Expression of Osteopontin in ALS-resistant Motor Neurons is a Critical Determinant of Late Phase Neurodegeneration Mediated by Matrix Metalloproteinase-9 Authors: Yuta Morisaki Sci Rep, 2016;6(0):27354. Species: Mouse Sample Type: Whole Tissue Application: IHC - PFA fixed Intracellular osteopontin stabilizes TRAF3 to positively regulate innate antiviral response Authors: K Zhao, M Zhang, L Zhang, P Wang, G Song, B Liu, H Wu, Z Yin, C Gao Sci Rep, 2016;6(0):23771. Species: Mouse Sample Type: recombinant protein Application: Neut Alternatively activated macrophages determine repair of the infarcted adult murine heart J Clin Invest, 2016;0(0):. Species: Mouse Sample Type: Whole Cells Application: Neut Cutting edge: Role of osteopontin and integrin alphav in T cell-mediated anti-inflammatory responses in endotoxemia. Authors: Inoue M, Shinohara M J Immunol, 2015;194(12):5595-8. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Capture Identification of therapeutic targets for glioblastoma by network analysis. Authors: Friedmann-Morvinski D, Bhargava V, Gupta S, Verma I, Subramaniam S Oncogene, 2015;35(5):608-20. Species: Mouse Sample Type: Cell Lysates Application: WB Hepatocellular carcinoma originates from hepatocytes and not from the progenitor/biliary compartment. Authors: Mu X, Espanol-Suner R, Mederacke I, Affo S, Manco R, Sempoux C, Lemaigre F, Adili A, Yuan D, Weber A, Unger K, Heikenwalder M, Leclercq I, Schwabe R J Clin Invest, 2015;125(10):3891-903. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Osteopontin depletion decreases inflammation and gastric epithelial proliferation during Helicobacter pylori infection in mice. Authors: Park J, Lee S, Go D, Kim H, Kwon H, Kim D Lab Invest, 2015;95(6):660-71. Species: Mouse Sample Type: Cell Lysates Application: WB Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing. Authors: Usoskin D, Furlan A, Islam S, Abdo H, Lonnerberg P, Lou D, Hjerling-Leffler J, Haeggstrom J, Kharchenko O, Kharchenko P, Linnarsson S, Ernfors P Nat Neurosci, 2015;18(1):145-53. Species: Mouse Sample Type: Whole Tissue Application: IHC Loss of JUNB/AP-1 promotes invasive prostate cancer. Authors: Thomsen M, Bakiri L, Hasenfuss S, Wu H, Morente M, Wagner E Cell Death Differ, 2015;22(4):574-82. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Endothelial Notch activity promotes angiogenesis and osteogenesis in bone. Authors: Ramasamy S, Kusumbe A, Wang L, Adams R Nature, 2014;507(7492):376-80. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Impaired hepatocyte maturation, abnormal expression of biliary transcription factors and liver fibrosis in C/EBPalpha(Cebpa)-knockout mice. Authors: Akai Y, Oitate T, Koike T, Shiojiri N Histol Histopathol, 2014;29(1):107-25. Species: Mouse Sample Type: tissue Application: IHC - Not specified Colony-stimulating factor-1 signaling suppresses renal crystal formation. Authors: Taguchi K, Okada A, Kitamura H, Yasui T, Naiki T, Hamamoto S, Ando R, Mizuno K, Kawai N, Tozawa K, Asano K, Tanaka M, Miyoshi I, Kohri K J Am Soc Nephrol, 2014;25(8):1680-97. Species: Mouse Sample Type: Whole Cells Application: Neut Coupling of angiogenesis and osteogenesis by a specific vessel subtype in bone. Authors: Kusumbe A, Ramasamy S, Adams R Nature, 2014;507(7492):323-8. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen PGC-1alpha induces SPP1 to activate macrophages and orchestrate functional angiogenesis in skeletal muscle. Authors: Rowe G, Raghuram S, Jang C, Nagy J, Patten I, Goyal A, Chan M, Liu L, Jiang A, Spokes K, Beeler D, Dvorak H, Aird W, Arany Z Circ Res, 2014;115(5):504-17. Species: Mouse Sample Type: Whole Cells Application: Neut Identification of a clonally expanding haematopoietic compartment in bone marrow. Authors: Wang L, Benedito R, Bixel M, Zeuschner D, Stehling M, Savendahl L, Haigh J, Snippert H, Clevers H, Breier G, Kiefer F, Adams R EMBO J, 2013;32(2):219-30. Species: Mouse Sample Type: Whole Tissue Application: IHC Fluorescence Focal adhesion kinase regulates the localization and retention of pro-B cells in bone marrow microenvironments. Authors: Park S, Wolfram P, Canty K, Harley B, Nombela-Arrieta C, Pivarnik G, Manis J, Beggs H, Silberstein L J Immunol, 2013;190(3):1094-102. Species: Mouse Sample Type: tissue Application: IHC-OCT embedded Repression of osteocyte Wnt/ÃŽÂ²-catenin signaling is an early event in the progression of renal osteodystrophy. Authors: Sabbagh Y, Graciolli FG, OBrien S, Tang W, Dos Reis LM, Ryan S, Phillips L, Boulanger J, Song W, Bracken C, Liu S, Ledbetter S, Dechow P, Canziani ME, Carvalho AB, Jorgetti V, Moyses RM, Schiavi SC J. Bone Miner. Res., 2012;27(8):1757-72. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Cellular basis of tissue regeneration by omentum. Authors: Shah S, Lowery E, Braun RK PLoS ONE, 2012;7(6):e38368. Species: Mouse Sample Type: Whole Cells Application: ICC Vitamin D receptor agonists increase klotho and osteopontin while decreasing aortic calcification in mice with chronic kidney disease fed a high phosphate diet. Authors: Lau, Wei Ling, Leaf, Elizabet, Hu, Ming Cha, Takeno, Marc M, Kuro-o, Makoto, Moe, Orson W, Giachelli, Cecilia Kidney Int, 2012;82(12):1261-70. Species: Mouse Sample Type: tissue Application: IHC - Not specified The critical role of agrin in the hematopoietic stem cell niche. Authors: Mazzon C, Anselmo A, Cibella J, Soldani C, Destro A, Kim N, Roncalli M, Burden SJ, Dustin ML, Sarukhan A, Viola A Blood, 2011;118(10):2733-42. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Adenosine and osteopontin contribute to the development of chronic obstructive pulmonary disease. Authors: Schneider DJ, Lindsay JC, Zhou Y, Molina JG, Blackburn MR FASEB J., 2010;24(1):70-80. Species: Mouse Sample Type: Whole Cells Application: ICC Neutralization of osteopontin inhibits obesity-induced inflammation and insulin resistance. Authors: Kiefer FW, Zeyda M, Gollinger K, Pfau B, Neuhofer A, Weichhart T, Saemann MD, Geyeregger R, Schlederer M, Kenner L, Stulnig TM Diabetes, 2010;59(4):935-46. Species: Mouse Sample Type: In Vivo Application: In vivo Phosphate feeding induces arterial medial calcification in uremic mice: role of serum phosphorus, fibroblast growth factor-23, and osteopontin. Authors: El-Abbadi MM, Pai AS, Leaf EM, Yang HY, Bartley BA, Quan KK, Ingalls CM, Liao HW, Giachelli CM Kidney Int., 2009;75(12):1297-307. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Therapeutic silencing of an endogenous gene by siRNA cream in an arthritis model mouse. Authors: Takanashi M, Oikawa K, Sudo K, Tanaka M, Fujita K, Ishikawa A, Nakae S, Kaspar RL, Matsuzaki M, Kudo M, Kuroda M Gene Ther., 2009;16(8):982-9. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Fibroblast growth factor-2 regulates expression of osteopontin in periodontal ligament cells. Authors: Terashima Y, Shimabukuro Y, Terashima H, Ozasa M, Terakura M, Ikezawa K, Hashikawa T, Takedachi M, Oohara H, Yamada S, Murakami S J. Cell. Physiol., 2008;216(3):640-50. Species: Mouse Sample Type: Cell Lysates Application: WB Increased osteopontin expression in dendritic cells amplifies IL-17 production by CD4+ T cells in experimental autoimmune encephalomyelitis and in multiple sclerosis. Authors: Murugaiyan G, Mittal A, Weiner HL J. Immunol., 2008;181(11):7480-8. Species: Mouse Sample Type: Cell Lysates Application: WB In vivo osteopontin-induced macrophage accumulation is dependent on CD44 expression. Authors: Marcondes MC, Poling M, Watry DD, Hall D, Fox HS Cell. Immunol., 2008;254(1):56-62. Species: Mouse Sample Type: recombinant OPN Application: WB Polyomavirus middle T antigen induces the transcription of osteopontin, a gene important for the migration of transformed cells. Authors: Whalen KA, Weber GF, Benjamin TL, Schaffhausen BS J. Virol., 2008;82(10):4946-54. Species: Mouse Sample Type: Cell Lysates Application: WB Osteopontin has a crucial role in allergic airway disease through regulation of dendritic cell subsets. Authors: Xanthou G, Alissafi T, Semitekolou M, Simoes DC, Economidou E, Gaga M, Lambrecht BN, Lloyd CM, Panoutsakopoulou V Nat. Med., 2007;13(5):570-8. Species: Mouse Sample Type: In Vivo Application: In vivo Inhalation of ultrafine carbon particles triggers biphasic pro-inflammatory response in the mouse lung. Authors: Andre E, Stoeger T, Takenaka S, Bahnweg M, Ritter B, Karg E, Lentner B, Reinhard C, Schulz H, Wjst M Eur. Respir. J., 2006;28(2):275-85. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Aggregation of embryonic stem cells induces Nanog repression and primitive endoderm differentiation. Authors: Hamazaki T, Oka M, Yamanaka S, Terada N J. Cell. Sci., 2004;117(0):5681-6. Species: Human Sample Type: Whole Cells Application: ICC Lack of requirement of osteopontin for inflammation, bone erosion, and cartilage damage in the K/BxN model of autoantibody-mediated arthritis. Authors: Jacobs JP, Pettit AR, Shinohara ML, Jansson M, Cantor H, Gravallese EM, Mathis D, Benoist C Arthritis Rheum., 2004;50(8):2685-94. Species: Mouse Sample Type: Serum Application: ELISA Development
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse Osteopontin/OPN (R&D Systems, Catalog # 441-OP) Leu17-Asn294 (Glu99Gly) Accession # Q547B5
内毒素水平	<0.30 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Osteopontin (OPN, previously also referred to as transformation-associated secreted phosphoprotein, bone sialoprotein I, 2ar, 2B7, early T lymphocyte activation 1 protein, minopotin, calcium oxalate crystal growth inhibitor protein), is a secreted, highly acidic, calcium-binding, RGD-containing, phosphorylated glycoprotein originally isolated from bone matrix. Subsequently, OPN has been found in kidney, placenta, blood vessels and various tumor tissues. Many cell types (including macrophages, osteoclasts, activated T cells, fibroblasts, epithelial cells, vascular smooth muscle cells, and natural killer cells) can express OPN in response to activation by cytokines, growth factors or inflammatory mediators. Elevated expression of OPN has also been associated with numerous pathobiological conditions such as atherosclerotic plaques, renal tubulointerstitial fibrosis, granuloma formations in tuberculosis and silicosis, neointimal formation associated with balloon catheterization, metastasizing tumors, and cerebral ischemia. Mouse OPN cDNA encodes a 294 amino acid (aa) residue precursor protein with a 16 aa residue predicted signal peptide that is cleaved to yield a 278 aa residue mature protein with an integrin binding sequence (RGD), and N- and O-glycosylation sites. OPN has been shown to bind to different cell types through RGD-mediated interaction with the integrins alpha _vbeta₁, alpha _vbeta₃, alpha _vbeta₅, and non-RGD-mediated interaction with CD44 and the integrins alpha ₈beta₁ or alpha ₉beta₁. Functionally, OPN is chemotactic for macrophages, smooth muscle cells, endothelial cells and glial cells. OPN has also been shown to inhibit nitric oxide production and cytotoxicity by activated macrophages. Human, mouse, rat, pig and bovine OPN share from approximately 40-80% amino acid sequence identity. Osteopontin is a substrate for proteolytic cleavage by thrombin, enterokinase, MMP-3 and MMP-7. The functions of OPN in a variety of cell types were shown to be modified as a result of proteolytic cleavage (2, 3).
运输条件	Blue Ice
存放说明	4℃
参考文献	Ann. N.Y. Acad. Sci., vol. 760, 1995, Apr. 21. Senger, D.R. et al. (1996) Biochim. Biophys. Acta. 1314:13. Agnihotri, R. et al. (2001) J. Biol. Chem. 276:28261.

参考图片

Cell Adhesion Mediated by Osteopontin/OPN and Neutralization by Mouse Osteopontin/OPN Antibody. Recombinant Mouse Osteopontin/OPN (Catalog # 441-OP), immobilized onto a microplate, supports the adhesion of the HEK293 human embryonic kidney cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Mouse Osteopontin/OPN (2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Osteopontin/OPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF808). The ND₅₀ is typically 1-3 µg/mL.

Osteopontin/OPN in Mouse Thymus. Osteopontin/OPN was detected in perfusion fixed frozen sections of mouse thymus using Mouse Osteopontin/OPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF808) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.