Human cIAP-1 (HIAP-2) Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human cIAP-1 (HIAP-2) Affinity Purified Polyclonal Ab (25 UG)

货号： AF8181-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF8181-SP
别名	API1Hiap-2; apoptosis inhibitor 1; baculoviral IAP repeat containing 2; baculoviral IAP repeat-containing 2; baculoviral IAP repeat-containing protein 2; BIRC2; cIAP1; c-IAP1; hIAP2; HIAP-2; IAP homolog B; IAP2; IAP-2; Inhibitor of apoptosis protein 2; MIHB; MIHBHIAP2; NFR2-TRAF signalling complex protein; RING finger protein 48; RNF48hiap-2; TNFR2-TRAF-signaling complex protein 2
全称	Cellular Inhibitor of Apoptosis Protein 1
反应种属	Human
应用	Western Blot(0.5 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human cIAP-1/HIAP-2. Does not cross-react with recombinant human cIAP-2 or XIAP.
使用方法	Western Blot: 0.5 µg/mL Immunohistochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	329 (Human); 11797 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Cytotoxic activity of the amphibian ribonucleases onconase and r-amphinase on tumor cells from B cell lymphoproliferative disorders. Authors: Smolewski P, Witkowska M, Zwolinska M, Cebula-Obrzut B, Majchrzak A, Jeske A, Darzynkiewicz Z, Ardelt W, Ardelt B, Robak T Int J Oncol, 2014;45(1):419-25. Species: Human Sample Type: Whole Cells Application: Flow Doxorubicin overcomes resistance to drozitumab by antagonizing Inhibitor of Apoptosis Proteins (IAPs). Authors: Zinonos I, Labrinidis A, Liapis V, Hay S, Panagopoulos V, Denichilo M, Ponomarev V, Ingman W, Atkins G, Findlay D, Zannettino A, Evdokiou A Anticancer Res, 2014;34(12):7007-20. Species: Human Sample Type: Cell Lysate Application: WB Receptor-interacting protein 1 increases chemoresistance by maintaining inhibitor of apoptosis protein levels and reducing reactive oxygen species through a microRNA-146a-mediated catalase pathway. Authors: Wang Q, Chen W, Bai L, Chen W, Padilla M, Lin A, Shi S, Wang X, Lin Y J Biol Chem, 2014;289(9):5654-63. Species: Human Sample Type: Cell Lysates Application: IP A novel role for inhibitor of apoptosis (IAP) proteins as regulators of endothelial barrier function by mediating RhoA activation. Authors: Hornburger M, Mayer B, Leonhardt S, Willer E, Zahler S, Beyerle A, Rajalingam K, Vollmar A, Furst R FASEB J, 2013;28(4):1938-46. Species: Human Sample Type: Whole Cells Application: WB Role of Apollon in human melanoma resistance to antitumor agents that activate the intrinsic or the extrinsic apoptosis pathways. Clin. Cancer Res., 2012;18(12):3316-27. Species: Human Sample Type: Cell Lysates Application: Flow Dacarbazine and the agonistic TRAIL receptor-2 antibody lexatumumab induce synergistic anticancer effects in melanoma. Authors: Engesaeter B, Engebraaten O, Florenes V, Maelandsmo G PLoS ONE, 2012;7(9):e45492. Species: Human Sample Type: Cell Lysates Application: WB Role of melanoma inhibitor of apoptosis (ML-IAP) protein, a member of the baculoviral IAP repeat (BIR) domain family, in the regulation of C-RAF kinase and cell migration. Authors: Oberoi-Khanuja, Tripat K, Karreman, Christia, Larisch, Sarit, Rapp, Ulf R, Rajalingam, Krishnar J Biol Chem, 2012;287(34):28445-55. Species: Human Sample Type: Cell Lysates Application: WB Characterization of ML-IAP protein stability and physiological role in vivo. Authors: Varfolomeev, Eugene, Moradi, Elham, Dynek, Jasmin N, Zha, Jiping, Fedorova, Anna V, Deshayes, Kurt, Fairbrother, Wayne J, Newton, Kim, Le Couter, Jennifer, Vucic, Domagoj Biochem J, 2012;447(3):427-36. Species: Human Sample Type: Cell Lysates Application: WB TWEAK Induces Apoptosis through a Death-signaling Complex Comprising Receptor-interacting Protein 1 (RIP1), Fas-associated Death Domain (FADD), and Caspase-8. Authors: Ikner A, Ashkenazi A J. Biol. Chem., 2011;286(24):21546-54. Species: Human Sample Type: Cell Lysates Application: WB RIP1 is required for IAP inhibitor-mediated sensitization of childhood acute leukemia cells to chemotherapy-induced apoptosis. Authors: Loder S, Fakler M, Schoeneberger H, Cristofanon S, Leibacher J, Vanlangenakker N, Bertrand MJ, Vandenabeele P, Jeremias I, Debatin KM, Fulda S Leukemia, 2011;26(5):1020-9. Species: Human Sample Type: Cell Lysates Application: WB Rapid and profound potentiation of Apo2L/TRAIL-mediated cytotoxicity and apoptosis in thoracic cancer cells by the histone deacetylase inhibitor Trichostatin A: the essential role of the mitochondria-mediated caspase activation cascade. Authors: Reddy RM, Yeow WS, Chua A, Nguyen DM, Baras A, Ziauddin MF, Shamimi-Noori SM, Maxhimer JB, Schrump DS Apoptosis, 2007;12(1):55-71. Species: Human Sample Type: Cell Lysates Application: WB Enhanced ubiquitination of cytoskeletal proteins in pressure overloaded myocardium is accompanied by changes in specific E3 ligases. Authors: Balasubramanian S, Mani S, Shiraishi H, Johnston RK, Yamane K, Willey CD, Cooper G, Tuxworth WJ, Kuppuswamy D J. Mol. Cell. Cardiol., 2006;41(4):669-79. Species: Feline Sample Type: Tissue Homogenates Application: WB cIAP1 Localizes to the nuclear compartment and modulates the cell cycle. Authors: Samuel T, Okada K, Hyer M, Welsh K, Zapata JM, Reed JC Cancer Res., 2005;65(1):210-8. Species: Human Sample Type: Cell Lysates Application: WB Activation of NF-kappaB and upregulation of intracellular anti-apoptotic proteins via the IGF-1/Akt signaling in human multiple myeloma cells: therapeutic implications. Authors: Mitsiades CS, Mitsiades N, Poulaki V, Schlossman R, Akiyama M, Chauhan D, Hideshima T, Treon SP, Munshi NC, Richardson PG, Anderson KC Oncogene, 2002;21(37):5673-83. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human cIAP-1/HIAP-2 His2-Ser618 Accession # Q13490
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	cIAP-1 (also known as BIR2, MIHB and HIAP-2) is a member of the inhibitor of apoptosis (IAP) family of proteins that inhibit the proteolytic activity of mature caspases. cIAP-1 has 3 BIR (baculovirus inhibitor of apoptosis) domains, a RING finger domain, and a caspase recruitment domain (CARD). cIAP-1 inhibits caspases by interaction of the BIR domain with the active caspase. Caspase activity may be restored through interactions with the Reaper like motif on mitochondrial proteins such as SMAC/Diablo or HTRA-2/Omi. cIAP-1 is reported to be cleaved by caspases in fetal rat hepatocytes treated with TGF-beta.
运输条件	Blue Ice
存放说明	4℃
参考文献	Roy, N. et al. (1997) EMBO J. 23:6914. Deveraux, Q. et al. (1997) Nature 388:300. Deveraux, Q. and J. Reed (1999) Genes & Develop. 13:239. Herrera, B.et al. (2002) FEBS Letters 520:93.

参考图片

Detection of Human cIAP‑1/HIAP‑2 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with human cIAP-1 (lane 1), human cIAP-2 (lane 2), or non-transfected (lane 3). PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human cIAP‑1/HIAP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8181) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, lystates of HepG2 human hepatocellular carcinoma cell line and Jurkat human acute T cell leukemia cell line were included. A specific band for cIAP‑1/HIAP‑2 was detected at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

cIAP‑1/HIAP‑2 in Human Lymph Node. cIAP‑1/HIAP‑2 was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-Human
cIAP‑1/HIAP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8181) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

cIAP‑1/HIAP‑2 in Human Lymphoma. cIAP‑1/HIAP‑2 was detected in immersion fixed paraffin-embedded sections of human lymphoma using Goat Anti-Human cIAP‑1/HIAP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8181) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.