Human cIAP-2 (HIAP-1) Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human cIAP-2 (HIAP-1) Affinity Purified Polyclonal Ab (25 UG)

货号： AF8171-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF8171-SP
别名	AIP1; API2; BIRC3 baculoviral IAP repeat containing 3; BIRC3; CIAP2; c-IAP2; HAIP1; HIAP1; HIAP-1; MALT2; MIHC; RNF49
反应种属	Human
应用	Western Blot(0.5 µg/mL) Simple Western(5 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human cIAP‑2/HIAP‑1 in Western blots. In Western blots, less than 1% cross-reactivity with cIAP-1 is observed.
使用方法	Western Blot: 0.5 µg/mL Simple Western: 5 µg/mL Immunohistochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	330 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Pro-Apoptotic Activity of New Honokiol/Triphenylmethane Analogues in B-Cell Lymphoid Malignancies Molecules, 2016;21(8):. Species: Human Sample Type: Whole Cells Application: Flow USP11-dependent selective cIAP2 deubiquitylation and stabilization determine sensitivity to Smac mimetics. Authors: Lee E, Seong D, Seo J, Jeong M, Lee H, Song J Cell Death Differ, 2015;22(9):1463-76. Species: Human Sample Type: Cell Lysates Application: WB Doxorubicin overcomes resistance to drozitumab by antagonizing Inhibitor of Apoptosis Proteins (IAPs). Authors: Zinonos I, Labrinidis A, Liapis V, Hay S, Panagopoulos V, Denichilo M, Ponomarev V, Ingman W, Atkins G, Findlay D, Zannettino A, Evdokiou A Anticancer Res, 2014;34(12):7007-20. Species: Human Sample Type: Cell Lysate Application: WB Dacarbazine and the agonistic TRAIL receptor-2 antibody lexatumumab induce synergistic anticancer effects in melanoma. Authors: Engesaeter B, Engebraaten O, Florenes V, Maelandsmo G PLoS ONE, 2012;7(9):e45492. Species: Human Sample Type: Cell Lysates Application: WB cIAP1 Localizes to the nuclear compartment and modulates the cell cycle. Authors: Samuel T, Okada K, Hyer M, Welsh K, Zapata JM, Reed JC Cancer Res., 2005;65(1):210-8. Species: Human Sample Type: Cell Lysates Application: WB Survivin interacts with Smac/DIABLO in ovarian carcinoma cells but is redundant in Smac-mediated apoptosis. Authors: McNeish IA, Lopes R, Bell SJ, McKay TR, Fernandez M, Lockley M, Wheatley SP, Lemoine NR Exp. Cell Res., 2005;302(1):69-82. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human cIAP‑2/HIAP‑1 Asn2-Ser604 Accession # U45878
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	cIAP-2 (also known as MIHC and HIAP-1) is a member of the inhibitor of apoptosis (IAP) family of proteins that inhibit the proteolytic activity of mature caspases. cIAP-2 has 3 BIR (baculovirus inhibitor of apoptosis) domains, a RING finger domain, and a caspase recruitment domain (CARD). cIAP-2 inhibits caspases through the direct interaction of its BIR domain with the active caspase. Caspase activity may be restored through interactions with the Reaper like motif on mitochondrial proteins such as SMAC/Diablo or HtrA2/Omi. cIAP-2 is reported to be cleaved by HtrA2/Omi.
运输条件	Blue Ice
存放说明	4℃
参考文献	Roy, N. et al. (1997) EMBO J. 23:6914. Deveraux, Q. et al. (1997) Nature 388:300. Deveraux, Q. and J. Reed (1999) Genes & Develop. 13:239. Srinivasula, S.M. et al. (2003) J. Biol. Chem. 278:31469. Yang, Q-H. et al. (2003) Genes Dev. 17:1487.

参考图片

cIAP‑2/HIAP‑1 in Human Colon. cIAP‑2/HIAP‑1 was detected in immersion fixed paraffin-embedded sections of human colon using 15 µg/mL Goat Anti-Human cIAP‑2/HIAP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8171) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the nucleus in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human cIAP‑2/HIAP‑1 by Western Blot. Western blot shows lysates of Raji human Burkitts lymphoma cell line and Daudi human Burkitts lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human cIAP‑2/HIAP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8171) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for cIAP‑2/HIAP‑1 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.

Detection of Human cIAP‑2/HIAP‑1 by Simple Western^TM. Simple Western lane view shows lysates of Raji human Burkitts lymphoma cell line and Daudi human Burkitts lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for cIAP‑2/HIAP‑1 at approximately 70 kDa (as indicated) using 5 µg/mL of Goat Anti-Human cIAP‑2/HIAP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8171) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.