| 货号 | AF1211-SP |
| 别名 | GH receptor; GHBP; GHR; growth hormone binding protein; growth hormone receptor; serum binding protein; Somatotropin receptor | 全称 | Growth Hormone Receptor |
| 反应种属 | Rat |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects rat Growth Hormone R/GHR in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant mouse GHR and recombinant human GHR is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize Growth Hormone R/GHR-mediated inhibition of proliferation in the Nb2‑11 rat lymphoma cell line. The Neutralization Dose (ND50) is typically 0.075-0.3 µg/mL in the presence of 10 ng/mL Recombinant Rat Growth Hormone R/GHR Fc Chimera and 0.2 ng/mL Recombinant Human Growth Hormone. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2690 (Human); 14600 (Mouse); 25235 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant rat Growth Hormone R/GHR Phe19-Arg265 Accession # P16310 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Growth hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins and somatolactin (1, 2). It is synthesized primarily by somatotropes in the anterior pituitary and is released as an endocrine hormone. Other cells and tissues, including lymphoid tissues, can also produce GH (3). GH is a pleiotropic molecule which can act directly or indirectly via IGF-I, to regulate growth and metabolism as well as enhance T cell survival and thymic functions (1, 2, 4). GH exerts its biological actions by binding to the GH receptor (GHR) that is present in many cell types (1, 2). Rat GHR cDNA encodes a 638 amino acid (aa) residue type I transmembrane protein with a 18 aa signal peptide, a 247 aa extracellular domain, a 24 aa transmembrane domain and a 349 aa cytoplasmic domain. An alternatively spliced 297 aa isoform of rat GHR also exists. This 279 aa variant corresponds to the serum GH-binding protein and is identical in sequence to the extracellular domain of the transmembrane protein up to Glu262 (5). Ligation of GHR by GH has been shown to result in receptor dimerization and activation of the JAK/STAT signaling cascade (6). The soluble GHBP has been shown to interfere with GH signaling by competing with the transmembrane receptor of GH. Alternatively, the GHBP has also been shown to enhance GH action by slowing GH clearance (5, 7). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Growth Hormone R/GHR Inhibition of Growth Hormone-dependent Cell Proliferation and Neutralization by Rat Growth Hormone R/GHR Antibody. Recombinant Rat Growth Hormone R/GHR Fc Chimera (Catalog # 1211‑GR) inhibits Recombinant Human Growth Hormone (Catalog # 1067‑GH) induced proliferation in the Nb2‑11 rat lymphoma cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human Growth Hormone (0.2 ng/mL) activity elicited by Recombinant Rat Growth Hormone R/GHR Fc Chimera (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat Growth Hormone R/GHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1211). The ND50 is typically 0.075‑0.3 µg/mL. |
