Human Growth Hormone (GH) Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human Growth Hormone (GH) Affinity Purified Polyclonal Ab (25 UG)

货号： AF1067-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF1067-SP
别名	GH1; GHIGHD1B; GHNGrowth hormone; GH-Nsomatotropin; growth hormone 1Pituitary growth hormone; hGH-N; Somatotropin
反应种属	Human
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human Growth Hormone in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross-reactivity with recombinant rat Growth Hormone is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize Growth Hormone-induced proliferation in the Nb2‑11 rat lymphoma cell line. Gout, P.W. et al. (1980) Cancer Res. 40:2433. The Neutralization Dose (ND₅₀) is typically 1.5-7.5 ng/mL in the presence of 0.2 ng/mL Recombinant Human Growth Hormone.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2688 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Growth hormone is permissive for neoplastic colon growth Proc Natl Acad Sci USA, 2016;0(0):. Species: Human Sample Type: Cell Lysates Application: WB STAT3 upregulation in pituitary somatotroph adenomas induces growth hormone hypersecretion. Authors: Zhou, Cuiqi, Jiao, Yonghui, Wang, Renzhi, Ren, Song-Gua, Wawrowsky, Kolja, Melmed, Shlomo J Clin Invest, 2015;125(4):1692-702. Species: Human Sample Type: Cell Lysates Application: WB Troubleshooting PEG-hGH detection supporting pharmacokinetic evaluation in growth hormone deficient patients. Authors: Myler HA, McVay S, Kratzsch J J Pharmacol Toxicol Methods, 2010;61(2):92-7. Species: Human Sample Type: Serum Application: Electrochemiluminescent Assay Growth hormone is present in the human retina and vitreous fluid. Authors: Harvey S, Parker E, Macdonald I, Sanders EJ Neurosci. Lett., 2009;455(3):199-202. Species: Human Sample Type: Vitreous Humor Application: WB Shift of monocyte function toward cellular immunity during sleep. Authors: Lange T, Dimitrov S, Fehm HL, Westermann J, Born J Arch. Intern. Med., 2006;166(16):1695-700. Species: Human Sample Type: Whole Cells Application: Neut
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human Growth Hormone Phe27-Phe217 Accession # CAA23779
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Growth Hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins, and somatolactin (1, 2). It is synthesized primarily by somatotropes in the anterior pituitary and is stored in secretary granules. The pulsatile release of GH into circulation is regulated by the concerted actions of the hypothalamic hormones - GH-releasing hormone (GHRH) and somatostatin (SST) - as well as by signals from the periphery - ghrelin (3) and leptin (4). The human GH cDNA encodes a 217 amino acid (aa) residue precursor protein with a 26 aa putative signal peptide. By alternative splicing, at least four isoforms of GH have been identified (5). Human GH is a pleiotropic cytokine that exerts its biological actions by binding to the transmembrane GH receptor, which is present in many cell types (1, 2). GH stimulates the liver and other tissues to produce IGF-I, which regulates growth and metabolism. GH has also been shown to have direct effects on growth that is independent of IGF-I. GH, directly or indirectly via IGF-I, can act on B cells, T cells, NK cells, macrophages, and neutrophils to exert immunomodulatory activities (6). In addition, GH can act directly on various cell types to induce lipolysis, lactation, amino acid uptake, and protein synthesis (1, 2, 6).
运输条件	Blue Ice
存放说明	4℃
参考文献	Goffin, V. et al. (1996) Endocrine Rev. 17:385. Le Roith, D. et al. (2001) Endocrine Rev.,22:53. Kojima, K. et al. (1999) Nature, 402:656. Tannenbaum, G. et al. (1998) Endocrinol. 139:3871. Welniak, L.A. et al. (2002)J. Leukoc. Biol. 71:381.

参考图片

Cell Proliferation Induced by Growth Hormone and Neutralization by Human Growth Hormone Antibody. Recombinant Human Growth Hormone (Catalog # 1067-GH) stimulates proliferation in the Nb2‑11 rat lymphoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Growth Hormone (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Growth Hormone Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1067). The ND₅₀ is typically 1.5-7.5 ng/mL.

Growth Hormone in Human Pituitary. Growth Hormone was detected in immersion fixed paraffin-embedded sections of human pituitary using Goat Anti-Human Growth Hormone Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1067) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.