Human Cardiotrophin-1 (CT-1) Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human Cardiotrophin-1 (CT-1) Affinity Purified Polyclonal Ab (25 UG)

货号： AF612-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF612-SP
别名	cardiophin 1; cardiotrophin 1; cardiotrophin-1; CT-1CT1; CTF1
反应种属	Human
应用	Western Blot(0.1 µg/mL)
目标/特异性	Detects human Cardiotrophin‑1/CT‑1 in Western blots. In Western blots, approximately 50% cross-reactivity with recombinant mouse Cardiotrophin-1 is observed.
使用方法	Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize Cardiotrophin‑1/CT‑1-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 0.1‑0.3 µg/mL in the presence of 15 ng/mL Recombinant Human Cardiotrophin‑1/CT‑1.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1489 (Human); 13019 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. HIF-1-mediated up-regulation of cardiotrophin-1 is involved in the survival response of cardiomyocytes to hypoxia. Authors: Robador PA, San Jose G, Rodriguez C, Guadall A, Moreno MU, Beaumont J, Fortuno A, Diez J, Martinez-Gonzalez J, Zalba G Cardiovasc. Res., 2011;92(2):247-55. Species: Human Sample Type: Cell Lysates Application: WB Cardiotrophin-1 induces interleukin-6 synthesis in human monocytes. Authors: Fritzenwanger M, Meusel K, Foerster M, Kuethe F, Krack A, Figulla HR Cytokine, 2007;38(3):137-44. Species: Human Sample Type: Whole Cells Application: Neut Alterations in Janus kinase (JAK)-signal transducers and activators of transcription (STAT) signaling in patients with end-stage dilated cardiomyopathy. Authors: Podewski EK, Hilfiker-Kleiner D, Hilfiker A, Morawietz H, Lichtenberg A, Wollert KC, Drexler H Circulation, 2003;107(6):798-802. Species: Human Sample Type: Tissue Homogenates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human Cardiotrophin-1/CT-1 Ser2-Ala201 Accession # Q16619
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Cardiotrophin-1 (CT-1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). It was originally isolated based on its ability to induce cardiac myocyte hypertrophy in vitro. CT-1 has since been shown to be a pleiotrophic cytokine with overlapping actions with other IL-6 family members on a variety of cell types. Human CT-1 encodes a 201 amino acid (aa) residue protein that lacks a hydrophobic signal peptide. The mechanism of CT-1 release from cells is currently not understood. Human and mouse CT-1 share 80% aa sequence identity and exhibit cross-species activity. CT-1 is highly expressed in heart, skeletal muscle, liver, lung and kidney. Lower levels of CT-1 expression is also seen in testis and brain. CT-1 initiates downstream signaling pathways through the heterodimerization of gp130 and the LIF receptor beta subunit. A third alpha receptor subunit has been implicated in the receptor complex.
运输条件	Blue Ice
存放说明	4℃
参考文献	Pennica, D.et al. (1996) Cytokine and Growth Factor Reviews 7:81. Robledo, O. et al. (1997) J. Biol. Chem. 272:4855.

参考图片

Cell Proliferation Induced by Cardiotrophin‑1/CT‑1 and Neutralization by Human Cardiotrophin‑1/CT‑1 Antibody.

Recombinant Human Cardiotrophin‑1/CT‑1 (Catalog # 612-CD) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Cardiotrophin‑1/CT‑1 (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Cardiotrophin‑1/CT‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF612). The ND₅₀ is typically 0.1-0.3 µg/mL.