| 货号 | AF7939-SP |
| 别名 | cell surface retention sequence binding protein-1; Cell surface retention sequence-binding protein 1; CRSBP1; CRSBP-1; extracellular link domain containing 1; extracellular link domain-containing 1; Extracellular link domain-containing protein 1; HAR; Hyaluronic acid receptor; lymphatic vessel endothelial hyaluronan receptor 1; lymphatic vessel endothelial hyaluronic acid receptor 1; LYVE-1XLKD1; XLKD1 | 全称 | Lymphatic Vessel Endothelial Hyaluronan Receptor 1 |
| 反应种属 | Rat |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects rat LYVE-1 in direct ELISA and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse LYVE-1 is observed, and approximately 10% cross-reactivity with recombinant human LYVE-1 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10894 (Human); 114332 (Mouse); 293186 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant rat LYVE-1 Thr53-Thr259 Accession # NP_001099756 |
| 生物活性 | Rat |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | LYVE-1 (Lymphatic vessel endothelial hyaluronic acid receptor 1; also CRSBP-1) is a 58-64 kDa, monomeric, glycoprotein member of the Link protein superfamily of hyaluron-binding molecules. It has limited expression, being found on the cell surface of lymphatic endothelial cells, endothelial cells of lymphoid sinuses, nodal stromal cells, and macrophages plus dendritic cells. HA (hyaluronan) is a nonsulfated, freestanding, repeating disaccharide consisting of GlcA (glucuronic acid) in beta ‑linkage with GlcNAc (N-acetylglucosamine). It should not be confused with heparan, which is sulfated, protein-linked, and composed of repeating GlcA/IdoA and GlcNAc residues in both alpha - and beta -linkages. HA is ubiquitous and occupies space between collagen fibers. It undergoes both normal, and pathology-induced turnover, and presumably does so by binding to LYVE-1, CD44 and HARE on lymphatic endothelium. Ultimately, HA is transported to the liver and nodes where it undergoes degradation. This may be necessary as low MW HA is proinflammatory. LYVE-1 is also a receptor for PDGF-BB and VEGF-A, and LYVE-1 ligation apparently induces endothelial cell contraction with the opening of intercellular junctions. Based on mouse, rat LYVE-1 is synthesized as a 343 amino acid (aa) type I transmembrane protein that contains a very long signal sequence (aa 1-52). The extracellular region is likely to be 182 aa in length (aa 53-234) and contain one Link domain (aa 62‑210). LYVE-12 is likely maintained in a default "off mode" by undergoing sialylation, possibly at Thr83. Over aa 53-259, rat LYVE-1 shares 82% and 60% aa sequence identity with mouse and human LYVE-1, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Rat LYVE‑1 by Western Blot. Western blot shows lysates of rat lymph node tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Rat LYVE‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7939) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LYVE‑1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
LYVE‑1 in Rat Kidney. LYVE‑1 was detected in perfusion fixed frozen sections of rat kidney using Sheep Anti-Rat LYVE‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7939) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to glomeruli. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
