| 货号 | AF6154-SP |
| 别名 | ARMD11; bA218C14.4 (cystatin C); CST3; cystatin 3; cystatin C (amyloid angiopathy and cerebral hemorrhage); cystatin C; cystatin-3; cystatin-C; Gamma-trace; MGC117328; Neuroendocrine basic polypeptide; Post-gamma-globulin |
| 反应种属 | Rat |
| 应用 | Western Blot(1 µg/mL) Simple Western(10 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects rat Cystatin C in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant mouse Cystatin C and approximately 15% cross-reactivity with recombinant human Cystatin C is observed. |
| 使用方法 | Western Blot: 1 µg/mL Simple Western: 10 µg/mL Immunohistochemistry: 5-15 µg/mL Immunoprecipitation: 25 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1471 (Human); 13010 (Mouse); 25307 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant rat Cystatin C Met1-Ala140 Accession # P14841 |
| 生物活性 | Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Cystatin C is a member of family 2 in the Cystatin superfamily (1). It is involved in processes such as tumor invasion and metastasis, inflammation and some neurological diseases. It inhibits many cysteine proteases such as papain and cathepsins B, H, K, L and S (2, 3). It is ubiquitous in human tissues and body fluids. A point mutation in the gene coding for the 120 amino acid mature Cystatin C causes a hereditary form of amyloid angiopathy in which the protein variant is deposited in the cerebral arteries, leading to fatal cerebral hemorrhage (4). Cystatin C may have additional clinical applications. For example, it is a good marker for glomerular filtration rate (5). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Rat Cystatin C by Western Blot. Western blot shows rat plasma. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Rat Cystatin C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6154) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cystatin C at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
Cystatin C in Rat Brain. Cystatin C was detected in perfusion fixed frozen sections of rat brain (cerebellum) using Sheep Anti-Rat Cystatin C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6154) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurons in the molecular layer of the cerebellum. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. | |
Detection of Rat Cystatin C by Simple WesternTM. Simple Western lane view shows rat plasma, loaded at 0.2 mg/mL. A specific band was detected for Cystatin C at approximately 28 kDa (as indicated) using 10 µg/mL of Sheep Anti-Rat Cystatin C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6154) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
