Rat MuSK Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Rat MuSK Affinity Purified Polyclonal Ab (25 UG)

货号： AF562-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF562-SP
别名	EC 2.7.10; EC 2.7.10.1; MGC126323; MGC126324; muscle, skeletal, receptor tyrosine kinase; MuSK; skeletal receptor tyrosine-protein kinase
全称	Muscle-specific Receptor Tyrosine Kinase
反应种属	Rat
应用	Western Blot(0.1 µg/mL)
目标/特异性	Detects rat MuSK in direct ELISAs and Western blots.
使用方法	Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize MuSK-dependent acetylcholine receptor clustering on myotubes differentiated from the C2C12 mouse myoblast cell line [Ferns, M.J. et al. (1993) Neuron 11:491]. The Neutralization Dose (ND₅₀) is typically 0.5-2 µg/mL in the presence of 16 ng/mL Recombinant Rat Agrin.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	4593 (Human); 81725 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. MuSK myasthenia gravis IgG4 disrupts the interaction of LRP4 with MuSK but both IgG4 and IgG1-3 can disperse preformed agrin-independent AChR clusters. Authors: Koneczny, Inga, Cossins, Judith, Waters, Patrick, Beeson, David, Vincent, Angela PLoS ONE, 2013;8(11):e80695. Species: Human Sample Type: Cell Lysate Application: IP Immunocapture and identification of cell membrane protein antigenic targets of serum autoantibodies. Authors: Littleton E, Dreger M, Palace J Mol. Cell Proteomics, 2009;8(7):1688-96. Species: Human Sample Type: Cell Lysates Application: WB Mutations causing DOK7 congenital myasthenia ablate functional motifs in Dok-7. Authors: Hamuro J, Higuchi O, Okada K, Ueno M, Iemura S, Natsume T, Spearman H, Beeson D, Yamanashi Y J. Biol. Chem., 2007;283(9):5518-24. Species: Mouse Sample Type: Cell Culture Supernates Application: WB MuSK expressed in the brain mediates cholinergic responses, synaptic plasticity, and memory formation. Authors: Garcia-Osta A, Tsokas P, Pollonini G, Landau EM, Blitzer R, Alberini CM J. Neurosci., 2006;26(30):7919-32. Species: Mouse Sample Type: Cell Lysates Application: WB 14-3-3 gamma associates with muscle specific kinase and regulates synaptic gene transcription at vertebrate neuromuscular synapse. Authors: Strochlic L, Cartaud A, Mejat A, Grailhe R, Schaeffer L, Changeux JP, Cartaud J Proc. Natl. Acad. Sci. U.S.A., 2004;101(52):18189-94. Species: Rat Sample Type: Cell Lysates Application: IP Postsynaptic requirement for Abl kinases in assembly of the neuromuscular junction. Authors: Finn AJ, Feng G, Pendergast AM Nat. Neurosci., 2003;6(7):717-23. Species: Mouse Sample Type: Cell Lysates Application: IP
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant rat MuSK
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	MuSK (Muscle-Specific Kinase) is a receptor tyrosine kinase expressed in muscle that regulates the formation of the neuromuscular junction. It binds the heparin sulfate proteoglycan Agrin to promote Acetylcholine Receptor clustering.
运输条件	Blue Ice
存放说明	4℃
参考文献	Receptor Tyrosine Kinases (RTKs) Synaptic Proteins Wnt Receptors

参考图片

Acetylcholine Receptor Clustering Mediated by MuSK and Neutralization by Rat MuSK Antibody. Recombinant Rat Agrin (Catalog # 550-AG) induces MuSK-dependent acetylcholine receptor clustering on myotubes differentiated from the C2C12 mouse myoblast cell line in a dose-dependent manner (orange line). Acetylcholine Receptor Clustering elicited by Recombinant Rat Agrin (16 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat MuSK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF562). The ND₅₀ is typically 0.5-2 µg/mL.