Rat IL-7 R alpha/CD127 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Rat IL-7 R alpha/CD127 Affinity Purified Polyclonal Ab (25 UG)

货号： AF5607-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF5607-SP
别名	CD127 antigen; CD127; CD127ILRA; CDW127; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL7R; IL7RA; IL-7RA; IL-7R-alpha; interleukin 7 receptor alpha chain; interleukin 7 receptor isoform H5-6; interleukin 7 receptor; interleukin-7 receptor subunit alpha
全称	Interleukin 7 Receptor alpha
反应种属	Rat
应用	Western Blot(1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects rat IL-7 R alpha /CD127 in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant mouse IL-7 R alpha and less than 10% cross-reactivity with recombinant human IL-7 R alpha is observed.
使用方法	Western Blot: 1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3575 (Human); 16197 (Mouse); 294797 (Rat)
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant rat IL-7 R alpha /CD127 Glu21-Asp239 Accession # NP_001099888
生物活性	Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	IL-7 R alpha (IL-7 receptor alpha; also CD127) is a 60-70 kDa member of the type I cytokine receptor family of molecules. It is expressed on resting naïve and memory CD8+ T cells, DN thymocytes, pre-B cells, Th1 CD4+ T cells, dendritic cells and monocytes. IL-7 R alpha heterodimerizes with both the gamma c chain to create the IL-7 receptor, and the TSLPR subunit to generate the TSLP receptor. IL-7 R alpha participates in T cell differentiation, naïve T cell survival, and CD4+ thymocyte proliferation. Mature rat IL-7 R alpha is a 437 aa type I transmembrane glycoprotein. It contains a 219 aa extracellular region (aa 21-239) that possesses one type III fibronectin domain (aa 129‑222), and a 193 aa cytoplasmic tail that shows a key phosphorylation site at Tyr447. Over aa 21‑239, rat IL-7 R alpha shares 79% and 67% aa identity with mouse and human IL-7 R alpha, respectively.
运输条件	Blue Ice
存放说明	4℃
参考文献	B Cell Development and Differentiation B Cell Markers Common gamma Chain Receptor Family Group 1 ILCs Group 2 ILCs Hematopoietic Progenitor Cell Markers Human CD Marker Products Interactive Pathway: IL-7 Signaling Pathways Isolation, Culture, and Identification of Natural Killer Cells LTi Cells Lymphoid Lineage Markers Natural Killer T (NKT) Cells NCR+ILC3 NCR-ILC3 Receptors in the Jak/STAT Pathway Regulatory T Cells (Tregs) T Cell Cytokine Signaling

参考图片

Detection of Rat IL‑7 R alpha /CD127 by Western Blot. Western blot shows lysates of rat spleen tissue. PVDF Membrane was probed with 1 µg/mL of Rat IL‑7 R alpha /CD127 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5607) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IL‑7 R alpha /CD127 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of IL‑7 R alpha /CD127 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Allophycocyanin-conjugated Mouse Anti-Rat CD4 Antibody and either (A) Normal Sheep IgG Control (Catalog # 5-001-A) or (B) Sheep Anti-Rat IL‑7 R alpha /CD127 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5607) followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126).