Rat beta-NGF Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Rat beta-NGF Affinity Purified Polyclonal Ab (25 UG)

货号： AF-556-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-556-SP
别名	Beta nerve growth factor precursor; beta-nerve growth factor; betaNGF; beta-NGF; HSAN5; MGC161426; nerve growth factor (beta polypeptide); nerve growth factor, beta subunit; NGFB; NGFBMGC161428
全称	beta Nerve Growth Factor
反应种属	Rat
应用	Western Blot,Immunohistochemistry,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects rat beta -NGF in ELISAs and Western blots. In ELISAs, less than 0.1% cross-reactivity with recombinant human (rh) beta ‑NGF, rhBDNF, recombinant rat (rr) GDNF, rhCNTF, rrGDNF R alpha, rrCNTF, rhGDNF, rhNT-3 and rhNT‑4 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL ELISA Capture (Matched Antibody Pair): 0.2-0.8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize beta ‑NGF-induced proliferation in the TF‑1 human erythroleukemic cell line [Kitamura, T. et al. (1989) J. Cell Physiol. 140:323]. The Neutralization Dose (ND₅₀) is typically 0.02-0.1 µg/mL in the presence of 10 ng/mL Recombinant Rat beta ‑NGF.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	4803 (Human); 18049 (Mouse); 310738 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Keratinocyte expression of inflammatory mediators plays a crucial role in substance P-induced acute and chronic pain. J Neuroinflammation, 2012;9(0):181. Species: Rat Sample Type: Whole Tissue Application: IHC Frozen Bradykinin and nerve growth factor play pivotal roles in muscular mechanical hyperalgesia after exercise (delayed-onset muscle soreness). Authors: Murase S, Terazawa E, Queme F, Ota H, Matsuda T, Hirate K, Kozaki Y, Katanosaka K, Taguchi T, Urai H, Mizumura K J. Neurosci., 2010;30(10):3752-61. Species: Rat Sample Type: In Vivo Application: In vivo Electrical stimulation of sympathetic neurons induces autocrine/paracrine effects of NGF mediated by TrkA. Authors: Saygili E, Schauerte P, Kuppers F, Heck L, Weis J, Weber C, Schwinger RH, Hoffmann R, Schroder JW, Marx N, Rana OR J. Mol. Cell. Cardiol., 2010;49(1):79-87. Species: Rat Sample Type: Whole Cells Application: Neut
纯化方式	Antigen Affinity-purified
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant rat beta -NGF (R&D Systems, Catalog # 556-NG) Ser122-Gly241 Accession # P25427
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	NGF was initially isolated in the mouse submandibular gland over three decades ago as a 7S complex composed of three non-covalently linked subunits, alpha, beta, and gamma. It is now known that both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta subunit, called beta -NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. Recombinant rat beta -NGF is a homodimer of two 120-amino acid polypeptides. The human protein shares approximately 90% homology at the amino acid level with both the mouse and rat beta -NGF and exhibits cross-species activity. NGF is a well-characterized neurotrophic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor-dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system.
运输条件	Blue Ice
存放说明	4℃
参考文献	Airway Inflammation and Remodeling Developmental Factors Implicated in Neurodegeneration Diabetic Peripheral Neuropathy Neurotrophic Factors and Receptors Neurotrophin/Trk Family VPS10P-domain Family

参考图片

Cell Proliferation Induced by beta ‑NGF and Neutralization by Rat beta ‑NGF Antibody. Recombinant Rat beta ‑NGF (Catalog # 556-NG) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat beta ‑NGF (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat beta -NGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-556-NA). The ND₅₀ is typically 0.02-0.1 µg/mL.

beta -NGF in Rat Brain. beta -NGF was detected in perfusion fixed frozen sections of rat brain using Rat beta -NGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-556-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.