Rat IL-18/IL-1F4 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Rat IL-18/IL-1F4 Affinity Purified Polyclonal Ab (25 UG)

货号： AF521-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF521-SP
别名	Iboctadekin; IFN-gamma-inducing factor; IGIF; IGIFIL-1 gamma; IL-18MGC12320; IL-1F4; IL1F4iboctadekin; IL-1g; Interferon gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; interleukin-18
全称	Interleukin 18
反应种属	Rat
应用	Western Blot(0.1 µg/mL)
目标/特异性	Detects rat IL‑18/IL-1F4 in direct ELISAs and Western blots. In Western blots, approximately 35% cross-reactivity with recombinant mouse IL‑18 is observed and approximately 10% cross-reactivity with recombinant human IL‑18 is observed.
使用方法	Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize IL‑18/IL‑1F4-induced IFN‑ gamma secretion in activated mouse T cells [Ahn, H.J. et al. (1997) J. Immunol. 159:2125]. The Neutralization Dose (ND₅₀) is typically 1-3 µg/mL in the presence of 15 ng/mL Recombinant Rat IL‑18/IL‑1F4 and 0.1 ng/mL Recombinant Mouse IL‑12.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Interleukin-18-mediated microglia/astrocyte interaction in the spinal cord enhances neuropathic pain processing after nerve injury. Authors: Miyoshi K, Obata K, Kondo T, Okamura H, Noguchi K J. Neurosci., 2008;28(48):12775-87. Species: Rat Sample Type: In Vivo Application: In vivo Inhibition of angiogenesis by interleukin-4 gene therapy in rat adjuvant-induced arthritis. Authors: Haas CS, Amin MA, Allen BB, Ruth JH, Haines GK, Woods JM, Koch AE Arthritis Rheum., 2006;54(8):2402-14. Species: Rat Sample Type: Tissue Homogenates Application: Neut Caspase-1-processed interleukins in hyperoxia-induced cell death in the developing brain. Authors: Felderhoff-Mueser U, Sifringer M, Polley O, Dzietko M, Leineweber B, Mahler L, Baier M, Bittigau P, Obladen M, Ikonomidou C, Buhrer C Ann. Neurol., 2005;57(1):50-9. Species: Rat Sample Type: Tissue Homogenates Application: WB Role of IL-18 in acute lung inflammation. Authors: Jordan JA, Guo RF, Yun EC, Sarma V, Warner RL, Crouch LD, Senaldi G, Ulich TR, Ward PA J. Immunol., 2001;167(12):7060-8. Species: Rat Sample Type: BALF Application: ELISA Development
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant rat IL‑18/IL-1F4 His37-Ser194 Accession # P97636
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Interleukin-18 (IL-18), also known as IL-1F4 and IFN-gamma inducing factor (IGIF), is a member of the IL-1 family of cytokines and is a key molecule in the innate immune response (1). Rat IL-18 is synthesized as a 24 kDa proprotein that contains a 36 amino acid (aa) propeptide and a 158 aa mature region (2). Under inflammatory conditions, the propeptide is cleaved by Caspase-1 in the cytoplasm to liberate the mature nonglycosylated 18 kDa monomeric IL-18 (3, 4). Mature rat IL-18 shares 91% aa sequence identity with mouse IL-18 and 60-64% aa sequence identity with human, canine, feline, porcine, and rhesus macaque IL-18. IL-18 is secreted by a variety of cell types including macrophages, dendritic cells, and epithelial cells (1, 5). Circulating mature IL-18 is sequestered by soluble IL-18 binding proteins (IL-18 BP) that inhibit IL-18 bioactivity (6). IL-18 interacts with the widely expressed IL-18 R alpha which then recruits the signaling subunit IL-18 R beta (7, 8). The IL-1 family member IL-1F7 also binds to IL-18 R alpha but does not recruit IL-18 R beta or induce signaling (9). IL-1F7 binds IL-18 BP and enhances its neutralizing effect on IL-18 activity (9). IL-18 synergizes with other cytokines to activate NK, Th1, and Th17 cells and to increase the production of IFN-gamma (1, 5, 10-12). IL-18 can also promote Th2 cytokine release which reduces the effectiveness of antiviral responses (13, 14). Increased levels of active IL-18 contribute to the severity of autoimmunity and hypertension, while deficiency of IL-18 results in symptoms of metabolic syndrome (1, 5, 15, 16). In cancer, IL-18 stimulates Th1 and NK cells to target tumor cells, but it can also promote angiogenesis, metastasis, and tumor cell immune evasion (11).
运输条件	Blue Ice
存放说明	4℃
参考文献	Arend, W.P. et al. (2008) Immunol. Rev. 223:20. Culhane, A.C. et al. (1998) Mol. Psychiatry 3:362. Ghayur, T. et al. (1997) Nature 386:619. Gu, Y. et al. (1997) Science 275:206. Boraschi, D. and C.A. Dinarello (2006) Eur. Cytokine Netw. 17:224. Novick, D. et al. (1999) Immunity 10:127. Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737. Born, T.L. et al. (1998) J. Biol. Chem. 273:29445. Bufler, P. et al. (2002) Proc. Natl. Acad. Sci. USA 99:13723. Takeda, K. et al. (1998) Immunity 8:383. Park, S. et al. (2007) Cell. Mol. Immunol. 4:329. Yoshimoto, T. et al. (1998) J. Immunol. 161:3400. Hoshino, T. et al. (2001) J. Immunol. 166:7014. Iannello, A. et al. (2009) AIDS Rev. 11:115. Rabkin, S.W. (2009) Nat. Clin. Pract. Cardiovasc. Med. 6:192. Netea, M.G. et al. (2006) Nat. Med. 12:650.

参考图片

IFN‑ gamma Secretion Induced by IL‑18/IL‑1F4 and Neutralization by Rat IL‑18/IL‑1F4 Antibody. In the presence of Recombinant Mouse IL‑12 (0.1 ng/mL, Catalog # 419‑ML), Recombinant Rat IL‑18/IL‑1F4 (Catalog # 521‑RL) stimulates IFN‑ gamma secretion in activated mouse T cells in a dose-dependent manner (orange line), as measured by the Mouse IFN‑ gamma Quantikine ELISA Kit (Catalog # MIF00). Under these conditions, IFN‑ gamma secretion elicited by Recombinant Rat IL‑18/IL‑1F4 (15 ng/mL) is neutralized (green line) by increasing concentrations of Rat IL‑18/IL‑1F4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF521). The ND₅₀ is typically 1-3 µg/mL.