Rat IL-1 beta/IL-1F2 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Rat IL-1 beta/IL-1F2 Affinity Purified Polyclonal Ab (25 UG)

货号： AF-501-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-501-SP
别名	catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta
全称	Interleukin 1 beta
反应种属	Rat
应用	Western Blot,Immunocytochemistry,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects rat IL-1 beta /IL-1F2 in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant mouse IL‑1 beta is observed and less than 0.5% cross-reactivity with recombinant human IL-1 beta and recombinant porcine IL-1 beta is observed.
使用方法	Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL ELISA Capture (Matched Antibody Pair): 0.2-0.8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND₅₀) is typically 2-10 µg/mL in the presence of 10 ng/mL Recombinant Rat IL‑1 beta /IL‑1F2 and 1.25 µg/mL concanavalin A.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Microglia-derived IL-1? promotes chemokine expression by Mï¿½ller cells and RPE in focal retinal degeneration Authors: R Natoli, N Fernando, M Madigan, JA Chu-Tan, K Valter, J Provis, M Rutar Mol Neurodegener, 2017;12(1):31. Species: Rat Sample Type: In Vivo Application: Neut Neuroinflammation increases GABAergic tone and impairs cognitive and motor function in hyperammonemia by increasing GAT-3 membrane expression. Reversal by sulforaphane by promoting M2 polarization of microglia Authors: V Hernandez-, A Cabrera-Pa, L Taoro-Gonz, A Gonzalez-U, A Agusti, T Balzano, M Llansola, V Felipo J Neuroinflammation, 2016;13(1):83. In Vitro Neuroprotective and Anti-Inflammatory Activities of Natural and Semi-Synthetic Spirosteroid Analogues Molecules, 2016;21(8):. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development Spatiotemporal Cadence of Macrophage Polarisation in a Model of Light-Induced Retinal Degeneration. Authors: Jiao H, Natoli R, Valter K, Provis J, Rutar M PLoS ONE, 2015;10(12):e0143952. Species: Rat Sample Type: Whole Tissue Application: IHC Frozen The spinal anti-inflammatory mechanism of motor cortex stimulation: cause of success and refractoriness in neuropathic pain? Authors: Silva G, Lopes P, Fonoff E, Pagano R J Neuroinflammation, 2015;12(0):10. Species: Rat Sample Type: Whole Tissue Application: IHC Not Specified Adenosine A2AR blockade prevents neuroinflammation-induced death of retinal ganglion cells caused by elevated pressure. Authors: Madeira M, Elvas F, Boia R, Goncalves F, Cunha R, Ambrosio A, Santiago A J Neuroinflammation, 2015;12(0):115. Species: Rat Sample Type: Tissue Culture Supernates Application: Neut Spinal cord injury induces a long-lasting upregulation of interleukin-1beta in astrocytes around the central canal. Authors: Paniagua-Torija B, Arevalo-Martin A, Molina-Holgado E, Molina-Holgado F, Garcia-Ovejero D Neuroscience, 2015;284(0):283-9. Species: Rat Sample Type: Whole Tissue Application: IHC Microglia enhance neurogenesis and oligodendrogenesis in the early postnatal subventricular zone. Authors: Shigemoto-Mogami Y, Hoshikawa K, Goldman J, Sekino Y, Sato K J Neurosci, 2014;34(6):2231-43. Species: Rat Sample Type: Whole Cells Application: Neut Prolonged neuroinflammation after lipopolysaccharide exposure in aged rats. Authors: Fu H, Yang T, Xiao W, Fan L, Wu Y, Terrando N, Wang T PLoS ONE, 2014;9(8):e106331. Species: Rat Sample Type: Whole Tissue Application: IHC Frozen Inflammatory mediators induced by amyloid-beta in the retina and RPE in vivo: implications for inflammasome activation in age-related macular degeneration. Authors: Liu R, Gao J, Cao S, Sandhu N, Cui J, Chou C, Fang E, Matsubara J Invest Ophthalmol Vis Sci, 2013;54(3):2225-37. Species: Rat Sample Type: Whole Tissue Application: IHC Paraffin-embedded Keratinocyte expression of inflammatory mediators plays a crucial role in substance P-induced acute and chronic pain. J Neuroinflammation, 2012;9(0):181. Species: Rat Sample Type: Whole Tissue Application: IHC Frozen Unmasking of LPA1 receptor-mediated migration response to lysophosphatidic acid by interleukin-1beta-induced attenuation of Rho signaling pathways in rat astrocytes. Authors: Sato K, Horiuchi Y, Jin Y, Malchinkhuu E, Komachi M, Kondo T, Okajima F J. Neurochem., 2011;117(1):164-74. Species: Rat Sample Type: Cell Lysates Application: WB Microglial cells contribute to endogenous brain defenses after acute neonatal focal stroke. Authors: Faustino JV, Wang X, Johnson CE J. Neurosci., 2011;31(36):12992-3001. Species: Rat Sample Type: Whole Tissue Application: IHC Galectin-1 attenuates astrogliosis-associated injuries and improves recovery of rats following focal cerebral ischemia. Authors: Qu WS, Wang YH, Ma JF, Tian DS, Zhang Q, Pan DJ, Yu ZY, Xie MJ, Wang JP, Wang W J. Neurochem., 2011;116(2):217-26. Species: Rat Sample Type: Whole Tissue Application: IHC Frozen Peripheral osmotic stimulation inhibits the brains innate immune response to microdialysis of acidic perfusion fluid adjacent to supraoptic nucleus. Authors: Summy-Long JY, Hu S Am. J. Physiol. Regul. Integr. Comp. Physiol., 2009;297(5):R1532-45. Species: Rat Sample Type: Whole Tissue Application: IHC Spinal leptin contributes to the pathogenesis of neuropathic pain in rodents. Authors: Lim G, Wang S, Zhang Y, Tian Y, Mao J J. Clin. Invest., 2009;119(2):295-304. Species: Rat Sample Type: Tissue Homogenates Application: WB Systemic immune challenge activates an intrinsically regulated local inflammatory circuit in the adrenal gland. Authors: Engstrom L, Rosen K, Angel A, Fyrberg A, Mackerlova L, Konsman JP, Engblom D, Blomqvist A Endocrinology, 2008;149(4):1436-50. Species: Rat Sample Type: Whole Tissue Application: IHC Frozen Rat pneumonia and soft-tissue infection models for the study of Acinetobacter baumannii biology. Authors: Russo TA, Beanan JM, Olson R, MacDonald U, Luke NR, Gill SR, Campagnari AA Infect. Immun., 2008;76(8):3577-86. Species: Rat Sample Type: BALF Application: ELISA Development Wnt-induced secreted protein-1 is a prohypertrophic and profibrotic growth factor. Authors: Colston JT, de la Rosa SD, Koehler M, Gonzales K, Mestril R, Freeman GL, Bailey SR, Chandrasekar B Am. J. Physiol. Heart Circ. Physiol., 2007;293(3):H1839-46. Species: Rat Sample Type: Whole Cells Application: Neut IL-1beta promotes neurite outgrowth by deactivating RhoA via p38 MAPK pathway. Authors: Temporin K, Tanaka H, Kuroda Y, Okada K, Yachi K, Moritomo H, Murase T, Yoshikawa H Biochem. Biophys. Res. Commun., 2007;365(2):375-80. Species: Rat Sample Type: Whole Cells Application: ICC Identification of astrocyte-expressed factors that modulate neural stem/progenitor cell differentiation. Authors: Barkho BZ, Song H, Aimone JB Stem Cells Dev., 2006;15(3):407-21. Species: Rat Sample Type: Whole Cells Application: Neut In vivo modulation of LPS-induced alterations in brain and peripheral cytokines and HPA axis activity by cannabinoids. Authors: Roche M, Diamond M, Finn DP J. Neuroimmunol., 2006;181(1):57-67. Species: Rat Sample Type: Plasma Application: ELISA Development Single subcutaneous administration of chorionic gonadotropin to rats induces a rapid and transient increase in testicular expression of pro-inflammatory cytokines. Authors: Assmus M, Svechnikov K, von Euler M, Setchell B, Sultana T, Zetterstrom C, Holst M, Soder O Pediatr. Res., 2005;57(6):896-901. Species: Rat Sample Type: Whole Tissue Application: IHC Ibuprofen protects ischemia-induced neuronal injury via up-regulating interleukin-1 receptor antagonist expression. Authors: Park EM, Cho BP, Volpe BT, Cruz MO, Joh TH, Cho S Neuroscience, 2005;132(3):625-31. Species: Rat Sample Type: Cell Lysates Application: WB A dual role for apolipoprotein e in neuroinflammation: anti- and pro-inflammatory activity. Authors: Guo L, LaDu MJ, Van Eldik LJ J. Mol. Neurosci., 2004;23(3):205-12. Species: Rat Sample Type: Cell Lysates Application: WB Differential cytokine response in interstitial fluid in skin and serum during experimental inflammation in rats. Authors: Nedrebo T, Reed RK, Jonsson R, Berg A, Wiig H J. Physiol. (Lond.), 2004;556(0):193-202. Species: Rat Sample Type: Whole Tissue Application: IHC Frozen Glucocorticoids play a fundamental role in protecting the brain during innate immune response. Authors: Nadeau S, Rivest S J. Neurosci., 2003;23(13):5536-44. Species: Rat Sample Type: In Vivo Application: In vivo
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant rat IL-1 beta /IL-1F2 (R&D Systems, Catalog # 501-RL) Val117-Ser268 Accession # Q63264
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 26% amino acid (aa) identity in rat. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL‑1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. The rat IL-1 beta cDNA encodes a 268 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine. The 17 kDa mature rat IL-1 beta shares 90% aa sequence identity with cotton rat and mouse and 65-77% with canine, equine, feline, human, porcine, and rhesus macaque IL-1 beta.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cancer Biomarkers Conventional/Classical Dendritic Cells Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis IL-1 Family Infectious Diseases Inflammatory Mediators Isolation, Culture, and Identification of Natural Killer Cells LTi Cells Macrophage Activation Markers Multiple Sclerosis and Myelin Related Molecules Myeloid-derived Suppressor Cells (MDSC) NCR-ILC3 Neuroinflammation NOD-like Receptors and the Inflammasome Platelets Regulatory T Cells (Tregs) T Cell Cytokine Signaling Th17 Cells Th9 Cells

参考图片

IL‑1 beta /IL‑1F2 in Rat Splenocytes. IL‑1 beta /IL‑1F2 was detected in immersion fixed LPS-stimulated rat splenocytes using 5 µg/mL Rat IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑501‑NA) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Rat IL‑1 beta /IL‑1F2 Antibody. Recombinant Rat IL‑1 beta /IL‑1F2 (Catalog # 501-RL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL‑1 beta /IL‑1F2 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-501-NA). The ND₅₀ is typically 2-10 µg/mL in the presence of concanavalin A (1.25 µg/mL).