| 货号 | AF1486-SP |
| 别名 | FABPI; fatty acid binding protein 2, intestinal; Fatty acid-binding protein 2; fatty acid-binding protein, intestinal; I-FABP; I-FABPMGC133132; Intestinal FABP; Intestinal-type fatty acid-binding protein | 全称 | Fatty Acid-Binding Protein 2/Intestinal FABP |
| 反应种属 | Rat |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects rat FABP2/I‑FABP in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant rat FABP1, recombinant human FABP3, recombinant mouse (rm) FABP4, and rmFABP5 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2169 (Human); 25598 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant rat FABP2/I‑FABP Met1-Glu132 Accession # P02693 |
| 生物活性 | Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | FABP2, also named I-FABP and gFABP, is a member of the intracellularfatty acid binding protein family. It is highly expressed in theintestine. FABP2 binds fatty acid in a non-covalent 1:1 complex tochaperone the lipids to cellular enzymes for metabolism and signaltransduction. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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FABP2/I‑FABP in Mouse Intestine. FABP2/I‑FABP was detected in immersion fixed frozen sections of adult mouse intestine using Goat Anti-Rat FABP2/I‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1486) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to intestinal epithelia. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections. | |
FABP2/I‑FABP in Rat Mesenchymal Stem Cells. FABP2/I‑FABP was detected in immersion fixed rat mesenchymal stem cells differentiated to adipocytes using Goat Anti-Rat FABP2/I‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1486) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips. |
