Rat FGF-BP Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Rat FGF-BP Affinity Purified Polyclonal Ab (25 UG)

货号： AF1413-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF1413-SP
别名	17 kDa heparin-binding growth factor-binding protein; FGF-BP; FGFBP1; FGF-BP1; FGFBP-1; FGFBPFGF-binding protein 1; fibroblast growth factor binding protein 1; fibroblast growth factor-binding protein 1; HBp17; HBP1717 kDa HBGF-binding protein; heparin-binding growth factor binding protein
全称	Fibroblast Growth Factor Binding Protein
反应种属	Rat
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects rat FGF-BP in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human FGF-BP is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Blockade of Receptor-ligand Interaction: In a functional ELISA, 0.5-2 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Rat FGF-BP (Catalog # 1413-FB) to immobilized Recombinant Human FGF-basic (Catalog # 233-FB) coated at 5 µg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	9982 (Human); 64535 (Rat)
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant rat FGF-BP Glu21-Cys238 Accession # Q9QY10
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Fibroblast growth factor binding protein (FGF-BP), also known as HBp17, is a secreted glycoprotein that increases the bioavailability of FGFs (1). Mature FGF-BP is a 34 kDa O-glycosylated protein with five conserved intrachain disulfide bonds (2-4). FGF-BP contains a heparin-binding domain (aa 113-146) and a distinct FGF-binding region (aa 197-238) (5). Mature rat FGF-BP shares 54% and 81% aa sequence identity with mouse and rat FGF-BP, respectively. FGF-BP is expressed throughout development and in adult squamous epithelium (6, 7). It is upregulated in injured skin, renal tubular epithelium, and spinal nerves as well as in carcinomas of the skin, colon, and pancreas (3, 8-11). FGF-BP binds FGF -1, -2, -7, -10, and -22 which are secreted and sequestered in the extracellular matrix (ECM) (8, 12). The interactions of FGF-BP with heparin sulfate proteoglycans (HSPG) and FGF, modulates their activities (7, 9 13, 14). FGF-BP enhances the mitogenic effects of FGFs, thereby contributing to epithelial, endothelial, and neuronal tissue repair, angiogenesis, and tumor growth (8-10, 12, 15, 16).
运输条件	Blue Ice
存放说明	4℃
参考文献	Abuharbeid, S. et al. (2006) Int. J. Biochem. Cell Biol. 38:1463. Aigner, A. et al. (2000) Growth Factors 18:51. Tassi, E. et al. (2006) Cancer Res.66:1191. Lametsch, R. et al. (2000) J. Biol. Chem. 275:19469. Xie, B. et al. (2006) J. Biol. Chem.281:1137. Aigner, A. et al. (2002) Histochem. Cell Biol.117:1. Wu, D. et al. (1991) J. Biol. Chem. 266:16778. Beer, H-D. et al. (2005) Oncogene24:5269. Ray, P.E. et al. (2006) Am. J. Physiol. Regul. Integr. Comp. Physiol. 290:R105. Tassi, E. et al. (2007) Am. J. Physiol. Regul. Integr. Comp. Physiol.293:R775. Kurtz, A. et al. (2004) Neoplasia6:595. Tassi, E. et al. (2001) J. Biol. Chem. 276:40247. Mongiat, M. et al. (2001) J. Biol. Chem. 276:10263. Kurtz, A. et al. (1997) Oncogene14:2671. Aigner, A. et al. (2001) Int. J. Cancer92:510. Czubayko, F. et al. (1997) Nat. Med.3:1137.

参考图片

FGF-BP in Rat Thymus. FGF-BP was detected in perfusion fixed frozen sections of rat thymus using Rat FGF-BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1413) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.