| 货号 | AF1116-SP |
| 别名 | CXCL7/Thymus Chemokine-1; Thymus Chemokine1 |
| 反应种属 | Rat |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects CXCL7/Thymus Chemokine‑1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse CXCL7 is observed and less than 1% cross-reactivity with _______________________ is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize CXCL7/Thymus Chemokine‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.8-4.0 µg/mL in the presence of 0.04 µg/mL Recombinant Rat CXCL7/Thymus Chemokine‑1. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 57349 (Mouse); 246358 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant rat CXCL7/Thymus Chemokine-1 Ile46-Ile107 Accession # NP_714943 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Thymus chemokine-1 (TCK-1) is a member of the CXC subfamily of chemokines. Mouse TCK-1 shares 72% amino acid sequence identity with rat TCK-1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Chemotaxis Induced by CXCL7/Thymus Chemokine‑1 and Neutralization by Rat CXCL7/Thymus Chemokine‑1 Antibody. Recombinant Rat CXCL7/Thymus Chemokine‑1 (Catalog # 1116-CK) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL7/Thymus Chemokine‑1 (0.04 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat CXCL7/Thymus Chemokine‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1116). The ND50 is typically 0.8-4.0 µg/mL. |
