| 货号 | AF985-SP |
| 别名 | IFG; IFI; IFNG; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma | 全称 | Interferon gamma |
| 反应种属 | Porcine |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects porcine IFN‑ gamma in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant canine IFN‑ gamma and recombinant feline IFN-gamma is observed, 10% cross-reactivity with recombinant equine IFN-gamma and recombinant human IFN-gamma is observed, and less than 5% cross-reactivity with recombinant mouse IFN-gamma, recombinant rat IFN-gamma, recombinant cotton rat IFN-gamma, and recombinant rhesus macaque IFN-gamma is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize IFN‑ gamma inhibition of EMCV-induced cytopathy in the PK‑15 porcine kidney epithelial cell line. The Neutralization Dose (ND50) is typically 0.6-3.0 µg/mL in the presence of 0.2 ng/mL Recombinant Porcine IFN‑ gamma. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant porcine IFN-gamma Ser21-Lys166 Accession # P17803 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Porcine |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature porcine IFN-gamma exists as a noncovalently linked homodimer of 20‑25 kDa variably glycosylated subunits (3). It shares 72%‑79% amino acid sequence identity with bovine, canine, equine, and feline IFN-gamma and 41%‑57% with cotton rat, human, mouse, rat, and rhesus IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI ( alpha subunits) which then interact with IFN-gamma RII ( beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, anti-proliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Porcine IFN‑ gamma Antibody. Recombinant Porcine IFN‑ gamma (Catalog # 985-PI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the PK‑15 porcine kidney epithelial cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Inhibition of EMCV activity elicited by Recombinant Porcine IFN‑ gamma (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF985). The ND50 is typically 0.6-3.0 µg/mL. |
