Canine IL-4 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Canine IL-4 Affinity Purified Polyclonal Ab (25 UG)

货号： AF754-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF754-SP
别名	B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL-4B_cell stimulatory factor 1; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra
全称	Interleukin 4
反应种属	Canine
应用	Western Blot(0.1 µg/mL)
目标/特异性	Detects canine IL-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human IL-4 is observed, 15% cross-reactivity with recombinant feline IL-4 and recombinant porcine IL-4 is observed and less than 1% cross-reactivity with recombinant cotton rat IL-4, recombinant mouse IL-4, and recombinant rat IL-4 is observed.
使用方法	Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize IL‑4-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 2-8 µg/mL in the presence of 50 ng/mL Recombinant Canine IL‑4.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Evidence for regulated interleukin-4 expression in chondrocyte-scaffolds under in vitro inflammatory conditions. Authors: Rai MF, Graeve T, Twardziok S, Schmidt MF PLoS ONE, 2011;6(10):e25749. Species: Canine Sample Type: Whole Cells Application: Neut
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant canine IL-4 His25-His132 Accession # O77762
内毒素水平	<0.01 EU per 1 μg of the antibody by the LAL method.
生物活性	Canine
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	IL-4 (Interleukin-4) is a cytokine that is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL-4 plays a dominant role in the development of allergic inflammation and asthma. The type I receptor for IL-4, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R? and the common ? chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R? and IL-13 R?1. The type II receptor also transduces IL-13 mediated signals.
运输条件	Blue Ice
存放说明	4℃
参考文献	BIObrief: Common gamma-Chain Family Cytokines Play a Pivotal Role in Regulating Immune System Functions Common gamma Chain Receptor Family Common gamma-Chain Family Cytokines Play a Pivotal Role in Regulating Immune System Functions Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis Cytokines and Receptors in Tfh Cell Differentiation and Function Dendritic Cell Development Eotaxin Stimulates Eosinophil Release of Preformed IL-4 gamma delta T Cells Group 2 ILCs ICC / IHC Images: IL-4 Inflammatory Mediators Interactive Pathway: IL-4 Signaling and its Primary Biological Effects in Different Immune Cell Types Interactive Pathway: IL-4 Signaling Pathways Multiple Sclerosis and Myelin Related Molecules Natural Killer T (NKT) Cells Regulatory T Cells (Tregs) T Cell Cytokine Signaling Th2 Cells Th9 Cells

参考图片

Cell Proliferation Induced by IL‑4 and Neutralization by Canine IL‑4 Antibody. Recombinant Canine IL‑4 (Catalog # 754-CL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL‑4 (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF754). The ND₅₀ is typically 2-8 µg/mL.