| 货号 | AF7080-SP |
| 别名 | CD8; CD8A |
| 应用 | Flow Cytometry(2.5 µg/106cells) |
| 目标/特异性 | Detects cotton rat CD8 alpha in direct ELISAs. In direct ELISAs, approximately 45% cross-reactivity with recombinant canine CD8, approximately 17% cross-reactivity with recombinant guinea pig CD8 alpha, and less than 2% cross-reactivity with recombinant mouse CD8 alpha is observed. |
| 使用方法 | Flow Cytometry: 2.5 µg/106cells |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 925 (Human); 12525 (Mouse); 24930 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant cotton rat CD8 alpha Ser24-Asp182 Accession # AAL55392 |
| 生物活性 | Cotton Rat |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | CD8 alpha (CD8 alpha ; also OX8, Leu-2 and Lyt-2) is a 23 kDa (predicted) member of the Ig superfamily of molecules. It is found on immature thymocytes, cytotoxic and suppressor T cells, select mast cells, and splenic plus bone marrow dendritic cells (in rodent). On T cells, CD8 alpha exists as either a disulfide linked homodimer, or heterodimer bound to CD8 beta. CD8 alpha beta is best known as a coreceptor for the TCR, enhancing TCR signaling. CD8 alpha alpha serves a different function and acts as a TCR corepressor that blocks T cell activation. Based on rat, mature Sigmodon hispidus/cotton rat CD8 alpha is a 210 amino acid (aa) type I transmembrane protein. It will possess a 161 aa extracellular region (aa 24-184) that contains one V-type Ig-like domain (aa 35-135), and a 30 aa cytoplasmic tail (aa 206-235). Although Sigmodon hispidus is called a rat, it is not. It is a rodent, and rat CD8 alpha is the closest ortholog to cotton rat CD8 alpha currently reported. Over the extracellular region, rat and cotton rat CD8 alpha share only 54% aa identity. Rat CD8 alpha runs at 34-39 kDa in SDS‑PAGE, and possesses one utilized N-linked and O-linked glycosylation site (vs. no N-linked sites in cotton rat CD8 alpha ). Based on this organization, and assuming O-linked glycosylation, cotton rat CD8 alpha would be expected to run at about 30-34 kDa in SDS‑PAGE. Over aa 24-182, cotton rat CD8 alpha shares 54% and 48% aa identity with rat and mouse CD8 alpha, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of CD8 in Cotton Rat Splenocytes by Flow Cytometry. Cotton rat splenocytes were stained with Sheep Anti-Cotton Rat CD8 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7080, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127). |
