| 货号 | AF693-SP |
| 别名 | CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF | 全称 | Interleukin 10 |
| 反应种属 | Porcine |
| 应用 | Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects porcine IL-10 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinat human IL-10, recombinant canine IL-10, recombinant cotton rat IL-10 and recombinant viral IL-10 is observed, 20% cross-reactivity with recombinant mouse IL‑10, recombinant feline IL-10 and recombinant equine IL-10 is observed and less than 5% cross-reactivity with recombinant rat IL-10 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant porcine IL-10 Ser19-Asn175 Accession # Q29055 |
| 生物活性 | Porcine |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | IL-10, initially designated cytokine synthesis inhibitory factor (CSIF), was originally identified as a product of murine T helper 2 (Th2) clones that inhibited the cytokine production by Th1 clones which are dependent upon stimulation with antigen in the presence of antigen presenting cells (APC). The human homologue of murine IL-10 was subsequently cloned by cross-hybridization. Human and murine IL-10 are 81% and 73% homologous at the nucleotide and amino acid level, respectively. Both murine and human IL-10 are also highly homologous with a previously uncharacterized open reading frame in the Epstein-Bar virus (EBV) genome, BCRF1. The BCRF1 gene product is now designated viral IL-10. Murine IL-10 is produced by Th2 cells, activated fetal thymocytes, macrophages, keratinocytes, and LY-1+(CD5+) and normal B cells. Human IL-10 has cross-species actvities and is active on mouse cells. Murine IL-10 is species-specific and does not act on human cells. Porcine IL-10 is 71% and 78% homologous with mouse and human IL-10, respectively. IL-10 is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is a potent immunosuppressant of macrophage functions. In vitro, IL-10 can inhibit the accessory function and antigen-presenting capacity of monocytes by, among other effects, down‑regulating class II MHC expression. Thus, IL-10 can inhibit monocyte/macrophage-dependent, antigen stimulated cytokine synthesis (especially IFN-gamma ) by human PBMNC and NK, and mouse Th1 cells. Additionally, IL-10 is a potent inhibitor of monocyte/macrophage activation and its resultant cytotoxic effects. As an immunostimulatory cytokine, IL-10 can act on B cells to enhance their viability, cell proliferation, Ig secretion, and class II MHC expression. Aside from B lymphocytes, IL-10 is also a growth co-stimulator for thymocytes and mast cells, as well as an enhancer of cytotoxic T cell development. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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IL‑10 in Porcine PBMCs. IL‑10 was detected in immersion fixed porcine peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Porcine IL‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF693) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
