| 货号 | AF681-SP |
| 别名 | catabolin; IL-1 beta; IL-1; IL1B; IL-1B; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta | 全称 | Interleukin 1 beta |
| 反应种属 | Porcine |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects porcine IL‑1 beta /IL‑1F2 in direct ELISAs and Western blots. In direct ELISAs, approximately 45%‑75% cross‑reactivity with recombinant human IL‑1 beta, recombinant mouse IL‑1 beta, recombinant rat IL‑1 beta, recombinant feline IL‑1 beta, recombinant canine IL‑1 beta, recombinant equine IL‑1 beta, recombinant guinea pig IL‑1 beta, recombinant rabbit IL‑1 beta, and recombinant rhesus monkey IL‑1 beta is observed and less than 15% cross-reactivity with recombinant cotton rat IL-1 beta is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J. A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 4-20 µg/mL in the presence of 20 ng/mL Recombinant Porcine IL‑1 beta /IL‑1F2 and 1.25 µg/mL concanavalin A. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Atorvastatin treatment improves survival and effects of implanted mesenchymal stem cells in post-infarct swine hearts. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant porcine IL‑1 beta /IL‑1F2 Ala115-Pro267 Accession # P26889 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Porcine |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 27% amino acid (aa) identity in porcine. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The porcine IL-1 beta cDNA encodes a 267 aa precursor. A 114 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature porcine IL-1 beta shares 63%-70% aa sequence identity with canine, cotton rat, equine, feline, human, mouse, rat, and rhesus IL-1 beta. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Porcine IL‑1 beta /IL‑1F2 Antibody. Recombinant Porcine IL‑1 beta /IL‑1F2 (Catalog # 681-PI) stimulates proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL‑1 beta /IL‑1F2 (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL‑1 beta / IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF681). The ND50 is typically 4‑20 µg/mL in the presence of concanavalin A (1.25 µg/mL). |
