Porcine IL-1 alpha/IL-1F1 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Porcine IL-1 alpha/IL-1F1 Affinity Purified Polyclonal Ab (25 UG)

货号： AF680-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF680-SP
别名	Hematopoietin-1; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; preinterleukin 1 alpha; pro-interleukin-1-alpha
全称	Interleukin 1 alpha
反应种属	Porcine
应用	Western Blot(0.1 µg/mL)
目标/特异性	Detects porcine IL‑1 alpha /IL‑1F1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human IL-1 alpha is observed and 10% cross-reactivity with recombinant mouse IL-1 alpha and recombinant rat IL-1 alpha is observed.
使用方法	Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize IL‑1 alpha /IL‑1F1-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J. A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND₅₀) is typically 0.006-0.012 µg/mL in the presence of 75 pg/mL Recombinant Porcine IL‑1 alpha /IL‑1F1 and 1.25 µg/mL concanavalin A.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Proteasome inhibition can impair caspase-8 activation upon submaximal stimulation of apoptotic tumor necrosis factor-related apoptosis inducing ligand (TRAIL) signaling. Authors: Laussmann MA, Passante E, Hellwig CT J. Biol. Chem., 2012;287(18):14402-11. Species: Human Sample Type: Cell Lysates Application: WB Induction of interleukin-1 in articular cartilage by explantation and cutting. Authors: Gruber J, Vincent TL, Hermansson M, Bolton M, Wait R, Saklatvala J Arthritis Rheum., 2004;50(8):2539-46. Species: Porcine Sample Type: Whole Cells Application: Neut
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant porcine IL-1 alpha Gln119-Ser270 Accession # P18430
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Porcine
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Interleukin 1 (IL-1) is a name that designates two proteins, IL-1 alpha and IL-1 beta, that are the products of distinct genes, but show approximately 25% amino acid sequence identity and recognize the same cell surface receptors. Although IL-1 production is generally considered to be a consequence of inflammation, evidence suggests that IL-1 is also temporarily upregulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. In response to stimuli produced by inflammatory agents, infections, or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cells is seen. Cells in particular known to produce IL-1 include osteoblasts, monocytes, macrophages, keratinocytes, Kupffer cells, hepatocytes, thymic and salivary gland epithelium, Schwann cells, fibroblasts and glia (oligodendroglia, astrocytes and microglia). IL-1 alpha and IL-1 beta are both synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17,000. Neither precursor contains a typical hydrophobic signal peptide sequence and most of the precursor form of IL-1 alpha remains in the cytosol of cells, although there is evidence for a membrane-bound form of the precursor form of IL-1 alpha. The IL-1 alpha precursor reportedly shows full biological activity in the EL-4 assay. Among various species, the amino acid sequence of mature IL-1 alpha is conserved 60% to 70% and porcine IL-1 has been found to be biologically active on murine cell lines. Both forms of IL-1 bind to the same receptors, designated as type I and type II. Evidence suggests that only the type I receptor is capable of signal transduction and that the type II receptor may function as a decoy, binding IL-1 and thus preventing the binding of IL-1 to the type I receptor.
运输条件	Blue Ice
存放说明	4℃
参考文献	Dower, S.K. and J.E. Sims, in Guidebook to Cytokines and their receptors (1994) N.A. Nicole ed. Oxford University Press NY p. 17.

参考图片

Cell Proliferation Induced by IL‑1 alpha /IL‑1F1 and Neutralization by Porcine IL‑1 alpha /IL‑1F1 Antibody. Recombinant Porcine IL‑1 alpha /IL‑1F1 (Catalog # 680-PI) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL‑1 alpha /IL‑1F1 (75 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL‑1 alpha /IL‑1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF680). The ND₅₀ is typically 0.006-0.012 µg/mL in the presence of concanavalin A (1.25 µg/mL).