Porcine IL-4 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Porcine IL-4 Affinity Purified Polyclonal Ab (25 UG)

货号： AF654-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF654-SP
别名	B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL-4B_cell stimulatory factor 1; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra
全称	Interleukin 4
反应种属	Porcine
应用	Western Blot,Immunocytochemistry,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects porcine IL-4 in ELISAs and Western blots. In ELISAs, this antibody shows less than 2% cross-reactivity with recombinant feline IL‑4 and less than 0.5% cross-reactivity with recombinant human IL‑4, recombinant canine IL‑4, recombinant cotton rat IL‑4, recombinant mouse IL‑4, and recombinant rat IL‑4.
使用方法	Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL ELISA Capture (Matched Antibody Pair): 0.2-0.8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize IL‑4-induced proliferation in the TF‑1 human erythroleukemic cell line. The Neutralization Dose (ND₅₀) is typically 1-5 µg/mL in the presence of 2 ng/mL Recombinant Porcine IL‑4.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Anti-inflammatory activity of nanocrystalline silver-derived solutions in porcine contact dermatitis. Authors: Nadworny PL, Wang J, Tredget EE J Inflamm (Lond), 2010;7(0):13. Species: Porcine Sample Type: Whole Tissue Application: IHC Paraffin-embedded Cytokine responses in gnotobiotic pigs after infection with virulent or attenuated human rotavirus. Authors: Azevedo MS, Yuan L, Pouly S, Gonzales AM, Jeong KI, Nguyen TV, Saif LJ J. Virol., 2006;80(1):372-82. Species: Porcine Sample Type: Whole Cells Application: ELISpot Development
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant porcine IL-4 His25-Cys133 Accession # Q04745
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Porcine
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Porcine IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Mature porcine IL-4 shares 78%, 59%, 41%, and 41% aa sequence identity with bovine, human, mouse, and rat IL-4, respectively. Human IL-4 is active on porcine vascular endothelial cells (5). IL-4 exerts its effects through two receptor complexes (6, 7). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on non-hematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4⁺ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgE in B cells, acquisition of the Th2 phenotype by naïve CD4⁺ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (8, 11). IL-4 plays a dominant role in the development of allergic inflammation and asthma (10, 12).
运输条件	Blue Ice
存放说明	4℃
参考文献	Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1. Bailey, M. et al. (1993) Biochim. Biophys. Acta 1171:328. Redfield, C. et al. (1991) Biochemistry 30:11029. Stocker, C.J. et al. (2000) J. Immunol. 164:3309. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701. Paludan, S.R. (1998) Scand. J. Immunol. 48:459. Corthay, A. (2006) Scand. J. Immunol. 64:93. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.

参考图片

Cell Proliferation Induced by IL‑4 and Neutralization by Porcine IL‑4 Antibody. Recombinant Porcine IL‑4 (Catalog # 654-P4) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL‑4 (2 ng/mL) is neutralized (green line) by increasing concentrations of Porcine IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF654). The ND₅₀ is typically 1‑5 µg/mL.

IL‑4 in Porcine PBMCs. IL‑4 was detected in immersion fixed porcine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Porcine IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF654) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.