| 货号 | AF6436-SP |
| 别名 | EC 3.2.1.35; HYA1; HYAL1; HYAL3; HYAL5; hyal-PH20; hyaluronidase PH-20; Hyaluronoglucosaminidase PH-20; MGC26532; PH20; PH-20; SPAG15; sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding); Sperm adhesion molecule 1; Sperm surface protein PH-20 | 全称 | Sperm Adhesion Molecule 1 |
| 反应种属 | Bovine |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects bovine SPAM1 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6677 (Human); 353352 (Bovine) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant bovine SPAM1 Leu36-Thr497 Accession # AAI10184 |
| 生物活性 | Bovine |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | Sperm adhesion molecule 1 (SPAM1), also known as PH-20 hyaluronidase (1), is encoded by one of the six hyaluronidase-like genes (1, 2, 3). SPAM1 is a GPI‑anchored enzyme located on the sperm surface and inner acrosomal membrane (4). SPAM1 degrades hyaluronic acid (HA), a major structural glycosaminoglycan found in extracellular matrices and basement membranes. The enzyme activity enables sperm to penetrate through the HA-rich cumulus cell layer surrounding the oocyte and therefore facilitates the fertilization process (5). However, detailed enzymatic analysis of this enzyme is hindered by the limited techniques/methods available to monitor the HA degradation products (6). A novel method for analyzing SPAM1 activity was utilized here. Because of the structural similarity between HA (repeating units of GlcA beta 1-3GlcNAc) and chondroitin sulfate (repeating units of GlcA beta 1-3GalNAc), the enzyme is also able to hydrolyze chondroitin sulfate. In this assay, radiolabeled chondroitin sulfate was digested with recombinant SPAM1. Degradation products were then separated using a polyacrylamide electrophoresis and visualized with an X-ray film (7). The bovine SPAM1 is 63% identical to human homologue in sequence. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Bovine SPAM1 by Western Blot. Western blot shows lysates of recombinant bovine SPAM1 (Catalog # 6436-GH). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Bovine SPAM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6436) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SPAM1 at approximately 78 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
