Canine CD25/IL-2 R alpha Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Canine CD25/IL-2 R alpha Affinity Purified Polyclonal Ab (25 UG)

货号： AF6227-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF6227-SP
别名	CD25 antigen; CD25; IDDM10; IL-2 R alpha; IL-2 receptor subunit alpha; IL-2R subunit alpha; IL2R; IL2RA; IL-2-RA; IL2-RA; interleukin 2 receptor, alpha; interleukin-2 receptor subunit alpha; p55; TAC antigen; TCGFR
全称	Interleukin 2 Receptor alpha
反应种属	Canine
应用	Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects canine CD25/IL-2 R alpha in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human CD25/IL-2 R alpha, recombinant mouse CD25/IL-2 R alpha, and recombinant rat CD25/IL-2 R alpha is observed.
使用方法	Flow Cytometry: 2.5 µg/10⁶cells
来源	Sterile PBS to a final concentration of 0.2 mg/mL.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine)
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant canine CD25/IL-2 R alpha Tyr19-Ile238 Accession # BAI49682
生物活性	Canine
标记	Unconjugated
溶解方法	Sterile PBS to a final concentration of 0.2 mg/mL.
背景	IL-2 receptor alpha (IL-2 R alpha ), also known as CD25, is a 55 kDa type I membrane glycoprotein that belongs to the family of cytokine receptors that utilize the common gamma chain subunit ( gamma _c). IL‑2 R alpha is primarily expressed on activated T cells and on regulatory T cells (Treg) (1‑3). The canine IL-2 R alpha cDNA encodes a 268 amino acid (aa) precursor that includes a 20 aa signal peptide, a 217 aa extracellular domain (ECD) with two Sushi domains, a 19 aa transmembrane segment, and an 12 aa cytoplasmic domain (4). Within the ECD, canine IL-2 R alpha shares 49% - 60% aa sequence identity with human, mouse, and rat IL-2 R alpha. IL-2 R beta (CD122) and gamma _c (IL‑2 R gamma /CD132) dimerize to form a constitutively expressed intermediate affinity IL-2 receptor (5, 6). By itself, IL-2 R alpha binds IL-2 with low affinity. It associates with IL-2 R beta and gamma _c to generate a ternary high affinity IL-2 receptor complex (7). A soluble form of IL‑2 R alpha can be generated by proteolytic cleavage of the cell surface receptor, rendering the T cell unresponsive to IL-2 (8, 9). Increased serum levels of soluble IL‑2 R alpha are found in some cancers and immune disorders (10). IL-2 R alpha is required for activation induced cell death (AICD) of naive T cells, a mechanism responsible for deleting autoreactive T cell clones (11, 12). IL-2 R alpha is also required for the development of CD4⁺CD25⁺ Treg which suppress autoreactive CD4⁺ T cells, thereby contributing to peripheral T cell homeostasis (11‑13).
运输条件	Blue Ice
存放说明	4℃
参考文献	Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245. Kovanen, P.E. and Leonard, W.J. (2004) Immunol. Rev. 202:67. Bluestone, J.A. and Tang, Q. (2005) Curr. Opin. Immunol. 17:638. Rissetto, K.C. et al. (2010) Vet. Immunol. Immunopathol. 135:137. Hatakeyama, M. et al. (1989) Science 244:551. Takeshita, T. et al. (1992) Science 257:379. Wang, X. et al. (2005) Science 310:1159. Wagner, D.K. et al. (1986) J. Immunol. 137:592. Schulz, O. et al. (1998) J. Exp. Med. 187:271. Witkowska, A.M. (2005) Mediat. Inflamm. 2005:121. Willerford, D.M. et al. (1995) Immunity 3:521. Van Parijs, L. et al. (1997) J. Immunol. 158:3738. Almeida, A.R.M. et al. (2002) J. Immunol. 169:4850.

参考图片

Detection of CD25/IL-2 R alpha in Canine Blood Monocytes by Flow Cytometry. Canine peripheral blood monocytes were stained with Sheep Anti-Canine CD25/IL‑2 R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6227) followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126) and Allophycocyanin-conjugated Anti-Canine CD4. Quadrant markers were set based on control antibody staining (Catalog # 5-001-A).