| 货号 | AF584-SP |
| 别名 | B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL-4B_cell stimulatory factor 1; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra | 全称 | Interleukin 4 |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects cotton rat IL‑4 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant rat IL-4 is observed, 5% cross-reactivity with recombinant mouse IL-4 is observed, and less than 1% cross-reactivity with recombinant human IL-4 and recombinant porcine IL-4 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize IL‑4-induced proliferation in the rat splenocytes. The Neutralization Dose (ND50) is typically 0.075-0.3 µg/mL in the presence of 10 ng/mL Recombinant Cotton Rat IL‑4 and 10 µg/mL PHA. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant cotton rat IL‑4 Cys21-Phe147 Accession # AAL18820 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Cotton Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa‑18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Cotton rat IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Mature cotton rat IL-4 shares 41%, 44%, 57%, and 68% aa sequence identity with bovine, human, mouse, and rat IL-4, respectively. IL-4 exerts its effects through two receptor complexes (5, 6). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgE in B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (7‑10). IL-4 plays a dominant role in the development of allergic inflammation and asthma (9, 11). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Cell Proliferation Induced by IL‑4 and Neutralization by Cotton Rat IL‑4 Antibody. Recombinant Cotton Rat IL‑4 (Catalog # 584-R4) stimulates proliferation in the rat splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Cotton Rat IL‑4 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF584). The ND50 is typically 0.075-0.3 µg/mL in the presence of PHA (10 µg/mL). |
